Influence of Extraction Protocol on Physical Properties of Parvovirus B19 DNA

While establishing a quantification standard for parvovirus B19 diagnostics, we extracted viral DNA from a high-titered human plasma by using three commercial kits. Despite similar viral DNA yields being obtained, striking differences in electrophoretic mobilities of the extracted nucleic acids were...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-12, Vol.48 (12), p.4595-4597
Main Authors: Matz, Bertfried, Kupfer, Bernd, Kreil, Thomas R, Eis-Hübinger, Anna Maria
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA, Viral - genetics
DNA, Viral - isolation & purification
Electrophoresis
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Parvovirus B19
Parvovirus B19, Human - genetics
Plasma - virology
Reagent Kits, Diagnostic
Specimen Handling - methods
Virology
Virology - methods
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Summary:While establishing a quantification standard for parvovirus B19 diagnostics, we extracted viral DNA from a high-titered human plasma by using three commercial kits. Despite similar viral DNA yields being obtained, striking differences in electrophoretic mobilities of the extracted nucleic acids were observed.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00571-10