Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification

To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tiss...

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Bibliographic Details
Published in:BioMed research international 2011-01, Vol.2011 (2011), p.1-10
Main Authors: Arai, Eri, Gotoh, Masahiro, Wakai-Ushijima, Saori, Hiraoka, Nobuyoshi, Kosuge, Tomoo, Hosoda, Fumie, Shibata, Tatsuhiro, Kondo, Tadashi, Yokoi, Sana, Imoto, Issei, Inazawa, Johji, Kanai, Yae
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Cancer
Carcinoma, Pancreatic Ductal - diagnosis
Carcinoma, Pancreatic Ductal - genetics
Care and treatment
Chromosomes, Artificial, Bacterial
Cohort Studies
CpG Islands
Deoxyribonucleic acid
Diagnosis
DNA
DNA Methylation
Female
Gene Amplification
Gene expression
Genetic aspects
Genome-Wide Association Study
Humans
Male
Methylation
Middle Aged
Multivariate Analysis
Oligonucleotide Array Sequence Analysis - methods
Pancreas
Pancreatic cancer
Pancreatic Neoplasms - diagnosis
Pancreatic Neoplasms - genetics
Polymerase Chain Reaction
Prognosis
Reproducibility of Results
Sensitivity and Specificity
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Summary:To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100%, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100% sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100% specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/780836