Conservation of polyamine regulation by translational frameshifting from yeast to mammals

• Published in: The EMBO journal 2000-04, Vol.19 (8), p.1907-1917
• Main Authors: Ivanov, I.P, Matsufuji, S, Murakami, Y, Gesteland, R.F, Atkins, J.F
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; antizyme; binding; binding proteins; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Conserved Sequence; enzyme inhibitors; enzymes; Evolution, Molecular; Frameshift Mutation; frameshifting; genbank/af217277; genbank/af217278; Gene Deletion; gene expression; genes; Humans; Mammals; messenger RNA; Molecular Sequence Data; Mutagenesis; Nematoda; nucleotide sequences; ornithine decarboxylase; polyamines; Polyamines - metabolism; Protein Biosynthesis; Proteins - chemistry; Proteins - genetics; Putrescine - metabolism; ribosomal frameshifting; ribosomes; Schizosaccharomyces - chemistry; Schizosaccharomyces pombe; Sequence Homology, Amino Acid; species differences; Spermidine - metabolism; Spermine - metabolism; Transcription, Genetic; translation; yeast; Yeasts
• ISSN: 0261-4189; 1460-2075; 1460-2075
• DOI: 10.1093/emboj/19.8.1907

Regulation of ornithine decarboxylase in vertebrates involves a negative feedback mechanism requiring the protein antizyme. Here we show that a similar mechanism exists in the fission yeast Schizosaccharomyces pombe . The expression of mammalian antizyme genes requires a specific +1 translational frameshift. The efficiency of the frameshift event reflects cellular polyamine levels creating the autoregulatory feedback loop. As shown here, the yeast antizyme gene and several newly identified antizyme genes from different nematodes also require a ribosomal frameshift event for their expression. Twelve nucleotides around the frameshift site are identical between S.pombe and the mammalian counterparts. The core element for this frameshifting is likely to have been present in the last common ancestor of yeast, nematodes and mammals.