Redox signaling: globalization of gene expression

Here we show that the extent of electron flow through the cbb 3 oxidase of Rhodobacter sphaeroides is inversely related to the expression levels of those photosynthesis genes that are under control of the PrrBA two‐component activation system: the greater the electron flow, the stronger the inhibito...

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Bibliographic Details
Published in:The EMBO journal 2000-08, Vol.19 (16), p.4237-4247
Main Authors: Oh II, Jeong, Kaplan, Samuel
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Bacterial Proteins
Blotting, Western
Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology
cbb3 cytochrome c oxidase
DNA-Binding Proteins - chemistry
Dose-Response Relationship, Drug
electron transport chain
Electron Transport Complex IV - chemistry
Electrons
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Enzymologic
gene regulation
Globalization
Ionophores - pharmacology
Light
Methacrylates
Models, Biological
Mutagenesis, Site-Directed
Operon
Oxidation-Reduction
Oxygen - metabolism
Photosynthesis
Photosynthesis - genetics
redox sensing
Repressor Proteins - chemistry
Rhodobacter sphaeroides
Rhodobacter sphaeroides - chemistry
Signal Transduction
Spectrophotometry
Thiazoles - pharmacology
Time Factors
Transcription, Genetic
Ultraviolet Rays
Uncoupling Agents - pharmacology
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Summary:Here we show that the extent of electron flow through the cbb 3 oxidase of Rhodobacter sphaeroides is inversely related to the expression levels of those photosynthesis genes that are under control of the PrrBA two‐component activation system: the greater the electron flow, the stronger the inhibitory signal generated by the cbb 3 oxidase to repress photosynthesis gene expression. Using site‐directed mutagenesis, we show that intramolecular electron transfer within the cbb 3 oxidase is involved in signal generation and transduction and this signal does not directly involve the intervention of molecular oxygen. In addition to the cbb 3 oxidase, the redox state of the quinone pool controls the transcription rate of the puc operon via the AppA–PpsR antirepressor–repressor system. Together, these interacting regulatory circuits are depicted in a model that permits us to understand the regulation by oxygen and light of photosynthesis gene expression in R.sphaeroides .
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1093/emboj/19.16.4237