The feasibility of ambulatory biosensor measurement of salivary alpha amylase: Relationships with self-reported and naturalistic psychological stress

• Published in: Biological psychology 2011-01, Vol.86 (1), p.50-56
• Main Authors: Robles, Theodore F., Shetty, Vivek, Zigler, Corwin M., Glover, Dorie A., Elashoff, David, Murphy, Debra, Yamaguchi, Masaki
• Format: Article
• Language: English
• Subjects: Adult; alpha-Amylases - metabolism; Behavioral psychophysiology; Biological and medical sciences; Biomarker; Biosensing Techniques - methods; Biosensor; Circadian Rhythm; Feasibility Studies; Fundamental and applied biological sciences. Psychology; Heart Rate; Humans; Male; Point of care measurement; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Regression Analysis; Saliva - chemistry; Salivary alpha amylase; Salivary diagnostics; Seasons; Self Report; Severity of Illness Index; Stress; Stress, Psychological - diagnosis; Stress, Psychological - metabolism; Time Factors; Young Adult
• ISSN: 0301-0511; 1873-6246
• DOI: 10.1016/j.biopsycho.2010.10.006

▶ A salivary alpha amylase biosensor can be deployed in naturalistic settings. ▶ Higher sAA levels were related to higher concurrent subjective distress. ▶ Higher depressive and isolation symptoms were related to lower sAA during exams. Recent developments in biosensor technology allow point-of-use reporting of salivary alpha amylase (sAA) levels while approaching the precision and accuracy of conventional laboratory-based testing. We deployed a portable prototype sAA biosensor in 54 healthy, male dental students during a low stress baseline and during final exams. At baseline, participants completed the Brief Symptom Inventory (BSI). At baseline and the exam week, participants provided saliva samples at 10 AM, 1 PM, and 5 PM, and rated concurrent subjective distress. Although subjective distress was higher during exams compared to baseline, sAA levels did not differ between baseline and exams. Higher sAA levels were related to higher concurrent subjective distress, and higher depressive and social isolation symptoms on the BSI were related to lower sAA during exams. Results from this study, in combination with previous validation data, suggest that the sAA biosensor is a promising tool for point-of-use measures of exposure to stress.