Low red blood cell levels of deglycating enzymes in colorectal cancer patients

• Published in: World journal of gastroenterology : WJG 2011-01, Vol.17 (3), p.329-333
• Main Authors: Notarnicola, Maria, Caruso, Maria Gabriella, Tutino, Valeria, Guerra, Vito, Misciagna, Giovanni
• Format: Article
• Language: English
• Subjects: Adult; Aged; Brief; Colorectal Neoplasms - blood; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - pathology; Erythrocytes - enzymology; Female; Humans; Lactoylglutathione Lyase - blood; Male; Middle Aged; Phosphotransferases (Alcohol Group Acceptor) - blood; 乙二醛; 大肠癌; 活性检测; 癌症患者; 红血细胞; 细胞水平; 辐射检测
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v17.i3.329

AIM： To investigate Glyoxalase Ⅰ and fructosamine-3-kinase （FN3K） activity in red blood cells from patients with colorectal adenomas and cancer. METHODS： Thirty three consecutive subjects with one or more histologically confirmed colorectal adenomatous polyps, 16 colorectal cancer patients and a group of 11 control subjects with normal colonoscopy were included in the study. Glyoxalase Ⅰ and FN3K activities were measured in red blood cells using a spectrophotometric and radiometric assay, respectively. RESULTS： A significant reduction in both Glyoxalase Ⅰ and FN3K activity was detected in patients with tumors compared to patients with adenomas and the controls. Erythrocyte Glyoxalase Ⅰ activity in colorectal cancer was approximately 6 times lower than that detected in patients with adenoma （0.022 ± 0.01 mmol/min per milliliter vs 0.128 ± 0.19 mmol/min per milliliter of red blood cells, P = 0.003, Tukey’s test）. FN3K activity in red blood cells from patients with colon cancer was approximately 2 times lower than that detected in adenoma patients （19.55 ± 6.4 pmol/min per milliliter vs 38.6 ± 31.7 pmol/min per milliliter of red blood cells, P = 0.04, Tukey’s test）. CONCLUSION： These findings suggest that deglycating enzymes may be involved in the malignant transformation of colon mucosa.