Identification of microRNAs associated with hyperthermia-induced cellular stress response

MicroRNAs (miRNAs) are a class of small RNAs that play a critical role in the coordination of fundamental cellular processes. Recent studies suggest that miRNAs participate in the cellular stress response (CSR), but their specific involvement remains unclear. In this study, we identify a group of th...

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Bibliographic Details
Published in:Cell stress & chaperones 2010-11, Vol.15 (6), p.1027-1038
Main Authors: Wilmink, Gerald J., Roth, Caleb L., Ibey, Bennett L., Ketchum, Norma, Bernhard, Joshua, Cerna, Cesario Z., Roach, William P.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Biology
Cell Line
Databases, Genetic
Eukaryotic Initiation Factor-2 - genetics
Eukaryotic Initiation Factor-2 - metabolism
Fibroblasts
Fibroblasts - metabolism
Gene expression regulation
Genes
Heat shock proteins
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Heat-Shock Response - genetics
Hot Temperature
Humans
Hyperthermia
Immunology
Messenger RNA
MicroRNA
MicroRNAs - metabolism
Neurosciences
Oligonucleotide Array Sequence Analysis
Oxidative stress
Polymerase Chain Reaction
RNA
SHORT COMMUNICATION
Stress response
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Summary:MicroRNAs (miRNAs) are a class of small RNAs that play a critical role in the coordination of fundamental cellular processes. Recent studies suggest that miRNAs participate in the cellular stress response (CSR), but their specific involvement remains unclear. In this study, we identify a group of thermally regulated miRNAs (TRMs) that are associated with the CSR. Using miRNA microarrays, we show that dermal fibroblasts differentially express 123 miRNAs when exposed to hyperthermia. Interestingly, only 27 of these miRNAs are annotated in the current Sanger registry. We validated the expression of the annotated miRNAs using qPCR techniques, and we found that the qPCR and microarray data was in well agreement. Computational target-prediction studies revealed that putative targets for the TRMs are heat shock proteins and Argonaute-2—the core functional unit of RNA silencing. These results indicate that cells express a specific group of miRNAs when exposed to hyperthermia, and these miRNAs may function in the regulation of the CSR. Future studies will be conducted to determine if other cells lines differentially express these miRNAs when exposed to hyperthermia.
ISSN:1355-8145
1466-1268
DOI:10.1007/s12192-010-0189-7