Curcumin induces maturation‐arrested dendritic cells that expand regulatory T cells in vitro and in vivo

Dendritic cells (DC) and regulatory T cells (Tregs) are vital to the development of transplant tolerance. Curcumin is a novel biological agent extracted from Curcuma longa (turmeric), with anti‐inflammatory and anti‐oxidant activity mediated via nuclear factor (NF)‐κB inhibition. We investigated the...

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Bibliographic Details
Published in:Clinical and experimental immunology 2010-12, Vol.162 (3), p.460-473
Main Authors: Rogers, N.M, Kireta, S, Coates, P.T.H
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antigens, CD - biosynthesis
Biological and medical sciences
Bystander Effect - drug effects
CD11c antigen
CD40 antigen
CD80 antigen
CD83 antigen
CD86 antigen
Cell Differentiation - drug effects
Cell Differentiation - immunology
Cell Proliferation - drug effects
Cells, Cultured
Curcuma - immunology
Curcuma longa
Curcumin
Curcumin - pharmacology
Dendritic cells
Dendritic Cells - drug effects
Dendritic Cells - immunology
Dendritic Cells - transplantation
Differentiation
Foxp3 protein
Fundamental and applied biological sciences. Psychology
gamma -Interferon
Gene expression
Humans
Immunological tolerance
Immunomodulation
Immunoregulation
Inflammation
Interleukins
Lipopolysaccharides
Lymphocyte Activation - drug effects
Lymphocyte Culture Test, Mixed
Lymphocytes T
Major histocompatibility complex
Male
maturation arrest
Mice
Mice, Inbred Strains
Monocytes
mRNA
regulatory T cells
RelB protein
T-Lymphocytes, Regulatory - immunology
Transcriptional Activation - drug effects
Translational Studies
Transplantation Tolerance
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Summary:Dendritic cells (DC) and regulatory T cells (Tregs) are vital to the development of transplant tolerance. Curcumin is a novel biological agent extracted from Curcuma longa (turmeric), with anti‐inflammatory and anti‐oxidant activity mediated via nuclear factor (NF)‐κB inhibition. We investigated the immunomodulatory effects of curcumin on human monocyte‐derived and murine DC. Human monocyte‐derived DC (hu‐Mo‐DC) were generated in the presence (CurcDC) or absence (matDC) of 25 µM curcumin, and matured using lipopolysaccharide (1 µg/ml). DC phenotype and allostimulatory capacity was assessed. CD11c⁺ DC were isolated from C57BL/6 mice, pretreated with curcumin and injected into BALB/c mice, followed by evaluation of in vivo T cell populations and alloproliferative response. Curcumin induced DC differentiation towards maturation‐arrest. CurcDC demonstrated minimal CD83 expression (
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2010.04232.x