Prophenoloxidase from Pieris rapae： gene cloning, activity, and transcription in response to venom/calyx fluid from the endoparasitoid wasp Cotesia glomerata

• Published in: Journal of Zhejiang University. B. Science 2011-02, Vol.12 (2), p.103-115
• Main Authors: Zhu, Jia-ying, Yang, Pu, Wu, Guo-xing
• Format: Article
• Language: English
• Subjects: Activation; Amino Acid Sequence; Amino acids; Animals; Base Sequence; Biomedical and Life Sciences; Biomedicine; Biotechnology; Catechol Oxidase - chemistry; Catechol Oxidase - genetics; Catechol Oxidase - metabolism; Cloning, Molecular; Cotesia glomerata; DNA Primers - genetics; DNA, Complementary - genetics; Down-Regulation - drug effects; Enzyme Precursors - chemistry; Enzyme Precursors - genetics; Enzyme Precursors - metabolism; Failure; Female; Fluid dynamics; Fluid flow; Fluids; Genes; Genes, Insect; Hemolymph - enzymology; Host-Parasite Interactions - genetics; Host-Parasite Interactions - immunology; Host-Parasite Interactions - physiology; Hymenoptera; Immunity, Innate; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Lepidoptera; Lepidoptera - enzymology; Lepidoptera - genetics; Lepidoptera - immunology; Lepidoptera - parasitology; Melanins - biosynthesis; Molecular Sequence Data; Phylogeny; Pieris rapae; Polyphenylene oxides; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Transcription, Genetic; Wasp Venoms - toxicity; Wasps - parasitology
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B1000275

Prophenoloxidase （PPO） plays an important role in melanization, necessary for defense against intruding parasitoids. Parasitoids have evolved to inject maternal virulence factors into the host hemocoel to suppress hemo- lymph melanization for the successful development of their progeny. In this study, the full-length complementary DNA （cDNA） of a Pieris rapae PPO was cloned. Its cDNA contained a 2076-base pair （bp） open reading frame （ORF） encoding 691 amino acids （aa）. Two putative copper-binding sites, a proteolytic activation site, three conserved hemocyanin domains, and a thiol ester motif were found in the deduced amino acid sequence. According to both multiple alignment and phylogenetic analysis, P. rapae PPO gene cloned here is a member of the lepidopteran PPO-2 family. Injection of Cotesia glomerata venom or calyx fluid resulted in reduction of P. rapae hemolymph phenoloxidase activity, demonstrating the ability to inhibit the host＇s melanization. Real-time reverse transcriptase polymerase chain reaction （RT-PCR） showed that transcripts of P. rapae PPO-2 in the haemocytes from larvae had not significantly changed following venom injection, suggesting that the regulation of PPO messenger RNA （mRNA） expression by venom was not employed by C. glomerata to cause failure of melanization in parasitized host. While decreased P. rapae PPO-2 gene expression was observed in the haemocytes after calyx fluid injection, no detectable transcriptional change was induced by parasitization, indicating that transcriptional down-regulation of PPO by calyx fluid might play a minor role involved in inhibiting the host＇s melanization.