Plasmodium vivax sporozoite production in Anopheles albimanus mosquitoes for vaccine clinical trials

Vaccine development for Plasmodium vivax malaria is underway. A model to assess the protective efficacy of vaccine candidates in humans is urgently needed. Given the lack of continuous P. vivax cultures, we developed a system to infect Anopheles albimanus mosquitoes using blood from P. vivax-infecte...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2011-02, Vol.84 (2 Suppl), p.28-34
Main Authors: Solarte, Yezid, Manzano, María R, Rocha, Leonardo, Hurtado, Hugo, James, Mark A, Arévalo-Herrera, Myriam, Herrera, Sócrates
Format: Article
Language:English
Subjects:
Animals
Anopheles - parasitology
Anopheles albimanus
Female
Humans
Insect Vectors - parasitology
Malaria Vaccines
Malaria, Vivax - blood
Malaria, Vivax - parasitology
Plasmodium vivax
Plasmodium vivax - physiology
Random Allocation
Sporozoites - physiology
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Summary:Vaccine development for Plasmodium vivax malaria is underway. A model to assess the protective efficacy of vaccine candidates in humans is urgently needed. Given the lack of continuous P. vivax cultures, we developed a system to infect Anopheles albimanus mosquitoes using blood from P. vivax-infected patients and determined parameters for challenge of malaria-naive volunteers by mosquito bite. Absence of co-infections in parasitized blood was confirmed by tests consistent with blood bank screening. A total of 119 experiments were conducted using batches of 900-4,500 mosquitoes fed by an artificial membrane feeding method. Optimal conditions for mosquito probing and infection were determined. Presence of oocyst and sporozoites were assessed on Days 7-8 and 14-15, respectively, and conditions to choose batches of infected mosquitoes for sporozoite challenge were established. Procedures to infect volunteers took a 2-hour period including verification of inoculum dose. Anopheles albimanus mosquitoes represent a valuable resource for P. vivax sporozoite challenge of volunteers.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2011.09-0499