Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometry method for determination of isoniazid in human plasma

An LC–MS/MS method for the determination of isoniazid in human plasma was developed and validated. Human plasma aliquots of 100 μL were used for analysis. The assay used nialamide as the internal standard. The calibration curve concentration range was 50–10,000 ng/mL. Sample preparation utilized pro...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-01, Vol.877 (3), p.285-290
Main Authors: Huang, Liusheng, Marzan, Florence, Jayewardene, Anura L., Lizak, Patricia S., Li, Xiaohua, Aweeka, Francesca T.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Antitubercular Agents - blood
Biological and medical sciences
Calibration
Chemical Phenomena
Chromatography, Liquid - methods
Drug Stability
Fundamental and applied biological sciences. Psychology
General pharmacology
Human plasma
Humans
Isoniazid
Isoniazid - blood
LC–MS/MS
Linear Models
Medical sciences
Method development
Nialamide
Nialamide - analysis
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Silica column
Tandem Mass Spectrometry - methods
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Summary:An LC–MS/MS method for the determination of isoniazid in human plasma was developed and validated. Human plasma aliquots of 100 μL were used for analysis. The assay used nialamide as the internal standard. The calibration curve concentration range was 50–10,000 ng/mL. Sample preparation utilized protein precipitation, and the supernatant was directly injected onto silica column without reconstitution. The recovery was over 90% and matrix effect was negligible. The method is simple and fast, which is advantageous in respect to instability of isoniazid in human plasma and loss on reconstitution due to its low molecular weight.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.12.024