RPLC Determination of Tinidazole and Diloxanide Furoate in Tablets

A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 0.2 M potassium dihydrogen phosphate (p...

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Bibliographic Details
Published in:Indian journal of pharmaceutical sciences 2008-09, Vol.70 (5), p.670-672
Main Authors: Pai, P N S, Rao, G K, Srinivas, B, Puranik, S
Format: Article
Language:English
Subjects:
Liquid chromatography
Measurement
Methods
Short Communication
Tinidazole
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Summary:A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 0.2 M potassium dihydrogen phosphate (pH 5) in the ratio 2:3:2.The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 282 nm. The described method was linear over a concentration range of 10-70 μg/ml and 10-90 μg/ml for the assay of diloxanide furoate and tinidazole, respectively. The mean recovery was found to be 100-101% for tinidazole and 97-103% for diloxanide furoate when determined at three different levels.
ISSN:0250-474X
1998-3743
DOI:10.4103/0250-474X.45415