Maturational growth of self-assembled, functional menisci as a result of TGF-β1 and enzymatic chondroitinase-ABC stimulation

Abstract Replacement of the knee meniscus requires a material possessing adequate geometrical and biomechanical properties. Meniscal tissue engineering attempts have been unable to produce tissue with collagen content and biomechanical properties, particularly tensile properties, mimicking native me...

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Bibliographic Details
Published in:Biomaterials 2011-03, Vol.32 (8), p.2052-2058
Main Authors: Huey, Daniel J, Athanasiou, Kyriacos A
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Biocompatible Materials - metabolism
Biomechanical Phenomena
C-ABC (chondroitinase-ABC)
Cartilage tissue engineering
Cartilage, Articular - cytology
Cartilage, Articular - physiology
Cattle
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - drug effects
Chondrocytes - physiology
Chondroitin ABC Lyase - metabolism
Chondroitin ABC Lyase - pharmacology
Collagen - metabolism
Compressive Strength
Dentistry
ECM
Knee meniscus
Materials Testing
Mechanical properties
Menisci, Tibial - anatomy & histology
Menisci, Tibial - drug effects
Menisci, Tibial - physiology
Tensile Strength
TGF (transforming growth factor)
Tissue Engineering - methods
Transforming Growth Factor beta1 - metabolism
Transforming Growth Factor beta1 - pharmacology
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Summary:Abstract Replacement of the knee meniscus requires a material possessing adequate geometrical and biomechanical properties. Meniscal tissue engineering attempts have been unable to produce tissue with collagen content and biomechanical properties, particularly tensile properties, mimicking native menisci. In an effort to obtain the geometric properties and the maturational growth necessary for the recapitulation of biochemical and, thus, biomechanical properties, a scaffoldless cell-based system, the self-assembly process, was used in conjunction with the catabolic enzyme chondroitinase-ABC and TGF-β1. We show that combinations of these agents resulted in maturational growth as evidenced by synergistic enhancement of the radial tensile modulus by 5-fold and the compressive relaxation modulus by 68%, and additive increases of the compressive instantaneous modulus by 136% and Col/WW by 196%. This study shows that tissue engineering can produce a biomaterial that is on par with the biochemical and biomechanical properties of native menisci.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2010.11.041