Circulating prosomatostatin-derived peptides: differential responses to food ingestion

Prosomatostatin (pro-S) and its bioactive posttranslational products, somatostatin-14 (S-14), somatostatin-13 (S-13), and somatostatin-28 (S-28), were measured in human plasma by the use of immunoglobulins to the NH2-terminus of S-28 conjugated with agarose to separate them and, thereafter, by RIA w...

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Bibliographic Details
Published in:The Journal of clinical investigation 1989-05, Vol.83 (5), p.1580-1589
Main Authors: ENSINCK, J. W, LASCHANSKY, E. C, VOGEL, R. E, SIMONOWITZ, D. A, ROOS, B. A, FRANCIS, B. H
Format: Article
Language:English
Subjects:
Adult
Aged
Amino Acid Sequence
Apud cells. Peptide and protein hormones. Growth factors
Arm - blood supply
Binding Sites, Antibody
Biological and medical sciences
Female
Food
Fundamental and applied biological sciences. Psychology
Gastrectomy
Humans
Immune Sera
Male
Mesenteric Veins
Middle Aged
Pancreatectomy
Peptide Fragments - blood
Portal Vein
Protein Precursors - blood
Protein Precursors - immunology
Somatostatin - blood
Somatostatin - immunology
Stomach - blood supply
Vertebrates: endocrinology
Citations: Items that cite this one
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Summary:Prosomatostatin (pro-S) and its bioactive posttranslational products, somatostatin-14 (S-14), somatostatin-13 (S-13), and somatostatin-28 (S-28), were measured in human plasma by the use of immunoglobulins to the NH2-terminus of S-28 conjugated with agarose to separate them and, thereafter, by RIA with an antiserum recognizing the COOH-terminus of pro-S, and by specific RIA for the NH2-terminus of S-14 and pro-S. In healthy men, mean basal levels of pro-S were 4 pg equivalent S-14/ml; S-14/S-13 combined were 9 pg equivalent S-14/ml; and S-28 levels were 16 pg/ml. After a 700-kcal meal, pro-S, S-14, and S-14/S-13 did not change, whereas S-28 levels doubled by 120 min and remained elevated for 240 min. To evaluate the origins of these peptides, their levels were compared in peripheral, portal, gastric, and mesenteric veins of anesthetized patients and in patients with total resection of stomach and pancreas before and after nutrient intake. The stomach and small intestine were sources of both peptides; however, most S-28 originated in the small intestine. These findings suggest that, in contrast to S-14, S-28 is a hormone and may modulate postprandial nutrient absorption and use.
ISSN:0021-9738
1558-8238
DOI:10.1172/JCI114055