Assessment of chemical species of lead accumulated in tidemarks of human articular cartilage by X-ray absorption near-edge structure analysis

A highly specific accumulation of the toxic element lead was recently measured in the transition zone between non‐calcified and calcified normal human articular cartilage. This transition zone, the so‐called `tidemark', is considered to be an active calcification front of great clinical importa...

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Published in:Journal of synchrotron radiation 2011-03, Vol.18 (2), p.238-244
Main Authors: Meirer, Florian, Pemmer, Bernhard, Pepponi, Giancarlo, Zoeger, Norbert, Wobrauschek, Peter, Sprio, Simone, Tampieri, Anna, Goettlicher, Joerg, Steininger, Ralph, Mangold, Stefan, Roschger, Paul, Berzlanovich, Andrea, Hofstaetter, Jochen G., Streli, Christina
Format: Article
Language:English
Subjects:
Cartilage, Articular - chemistry
Femur Head - chemistry
human bone
Humans
Lead - chemistry
Lead - metabolism
Patella - chemistry
Pb L3-edge XANES
Research Papers
tidemark
trabecular bone
X-ray absorption spectroscopy
X-Ray Absorption Spectroscopy - methods
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Summary:A highly specific accumulation of the toxic element lead was recently measured in the transition zone between non‐calcified and calcified normal human articular cartilage. This transition zone, the so‐called `tidemark', is considered to be an active calcification front of great clinical importance. However, little is known about the mechanisms of accumulation and the chemical form of Pb in calcified cartilage and bone. Using spatially resolved X‐ray absorption near‐edge structure analysis (µ‐XANES) at the Pb L3‐edge, the chemical state of Pb in the osteochondral region was investigated. The feasibility of the µ‐XANES set‐up at the SUL‐X beamline (ANKA synchrotron light source) was tested and confirmed by comparing XANES spectra of bulk Pb‐reference compounds recorded at both the XAS and the SUL‐X beamline at ANKA. The µ‐XANES set‐up was then used to investigate the tidemark region of human bone (two patella samples and one femoral head sample). The spectra recorded at the tidemark and at the trabecular bone were found to be highly correlated with the spectra of synthetic Pb‐doped carbonated hydroxyapatite, suggesting that in both of these very different tissues Pb is incorporated into the hydroxyapatite structure.
ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S0909049510052040