Stimulation of proliferation, differentiation, and function of human cells by primate interleukin 3

Cloned gibbon interleukin 3 (gIL-3) was found to stimulate the proliferation and differentiation of human bone marrow cells to produce day-14 granulocyte, macrophage, granulocyte-macrophage, and eosinophil colonies in semisolid agar. In the presence of normal human plasma, gIL-3 stimulated megakaryo...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (9), p.2761-2765
Main Authors: Lopez, A F, To, L B, Yang, Y C, Gamble, J R, Shannon, M F, Burns, G F, Dyson, P G, Juttner, C A, Clark, S, Vadas, M A
Format: Article
Language:English
Subjects:
Animals
Antibody-Dependent Cell Cytotoxicity - drug effects
Bone Marrow - drug effects
Bone Marrow Cells
Cell Differentiation - drug effects
Cell Division - drug effects
Cell Line
Chemotaxis, Leukocyte - drug effects
Chlorocebus aethiops
Eosinophils - cytology
Eosinophils - drug effects
Eosinophils - physiology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Humans
Interleukin-3 - pharmacology
Neutrophils - cytology
Neutrophils - drug effects
Neutrophils - immunology
Phagocytosis - drug effects
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Summary:Cloned gibbon interleukin 3 (gIL-3) was found to stimulate the proliferation and differentiation of human bone marrow cells to produce day-14 granulocyte, macrophage, granulocyte-macrophage, and eosinophil colonies in semisolid agar. In the presence of normal human plasma, gIL-3 stimulated megakaryocytes. In methylcellulose cultures, it stimulated erythroid colonies in the presence, but not in the absence, of erythropoietin. When mature human leukocytes were used, gIL-3 stimulated the function of purified mature eosinophils as measured by the capacity to kill antibody-coated target cells, to produce superoxide anions, and to phagocytize opsonized yeast particles in a manner similar to recombinant human granulocyte-macrophage colony-stimulating factor. In contrast, gIL-3 did not significantly stimulate any of the neutrophil functions tested, whereas human recombinant granulocyte-macrophage colony-stimulating factor was active in these assays. Among cytokines that are active on human hematopoietic cells, gIL-3 thus has a distinct set of functions and may predict the range of actions of the human molecule.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.9.2761