Mutation to Male Fertility and Toxin Insensitivity in Texas (T)-cytoplasm Maize is Associated with a Frameshift in a Mitochondrial Open Reading Frame

Tissue culture-derived mutants of male-sterile and disease toxin-sensitive Texas (T)-cytoplasm maize that exhibit male fertility and toxin insensitivity carry numerous alterations in mitochondrial DNA. In these mutants, a 6.7-kilobase Xho I fragment characteristic of parental T cytoplasm has been re...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (9), p.2858-2862
Main Authors: Wise, Roger P., Pring, Daryl R., Gengenbach, Burle G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences: Genetics
Classical genetics, quantitative genetics, hybrids
Corn
Cosmids
Cytoplasm
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Male fertility
Mitochondrial DNA
Open reading frames
Phenotypes
Pteridophyta, spermatophyta
Ribosomal DNA
RNA
Toxins
Vegetals
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Summary:Tissue culture-derived mutants of male-sterile and disease toxin-sensitive Texas (T)-cytoplasm maize that exhibit male fertility and toxin insensitivity carry numerous alterations in mitochondrial DNA. In these mutants, a 6.7-kilobase Xho I fragment characteristic of parental T cytoplasm has been rearranged. In the mutant T-4, the parental 6.7-kilobase Xho I fragment contains a guanine to adenine transition adjacent to a 5-base-pair insertion not found in T cytoplasm. The insertion, internal to a 345-base-pair open reading frame (T ORF13), generates a frameshift, resulting in a premature stop codon that terminates the open reading frame at base pair 222. In other mutants, the 345-base-pair ORF is part of a 3-kilobase deletion, which extends into a 5-kilobase repeat characteristic of mtDNA from T but not N male-fertile cytoplasm. Clones specific to T ORF13 hybridize to eight transcripts in T and T-4, yet only hybridize to three transcripts in T-7, a deletion mutant. Transcription of the T ORF13 region appears not to be altered in T-4, but the frameshift mutation in the T ORF13 reading frame indicates that a biologically inactive gene product could be associated with the mutational events. The results suggest that cytoplasmic male sterility and disease toxin sensitivity may be associated with presence of T ORF13 in T-cytoplasm maize.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.9.2858