Moisture sorption characteristics of freeze-dried human platelets

• Published in: Journal of Zhejiang University. B. Science 2011-03, Vol.12 (3), p.210-218
• Main Authors: Xu, Meng-jie, Chen, Guang-ming, Fan, Ju-li, Liu, Jin-hui, Xu, Xian-guo, Zhang, Shao-zhi
• Format: Article
• Language: English
• Subjects: Absorption; Biomedical and Life Sciences; Biomedicine; Biotechnology; Blood Platelets - cytology; Blood Preservation - instrumentation; Blood Preservation - methods; Diffusion coefficient; Equipment Design; Freeze Drying; Humans; Humidity; Materials Testing; Models, Statistical; Reproducibility of Results; Specimen Handling; Temperature; Water - chemistry
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B1010199

Freeze-drying is a promising method for a long-term storage of human platelets. The moisture sorption characteristics of freeze-dried human platelets (FDHPs) were studied in this paper. The moisture sorption isotherms of FDHPs and freeze-dried lyophilization buffer (FDLB) were measured at 4, 25, and 37 °C. The experimental data were fitted to Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) equations. There were no significant statistical differences ( P >0.05) between the sorption characteristics of FDHPs and FDLB at 4 and 25 °C, while FDHPs absorbed more water at 37 °C. The net isosteric heat of sorption was derived. The heat for FDHPs showed an abnormal negative value at low moisture contents when 25 and 37 °C data were used. Dynamic sorption experiments were carried out at 25 °C with environmental water activity controlled at 0.75, 0.85, and 0.90. The moisture diffusion coefficient was fitted to be 8.24×10 −12 m 2 /s when experimental data at initial time were used. These results would be helpful in choosing prehydration and storage condition for FDHPs.