Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy

To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518), in which a domain of Core and NS3 of hepatitis C virus (HCV) was fused, ELISA was performed in a total of 76 serum samples. Each serum was serially diluted using two-fold dilution method with...

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Bibliographic Details
Published in:Journal of Korean medical science 1999-04, Vol.14 (2), p.165-170
Main Authors: Park, Y M, Byun, B H, Choi, J Y, Bae, S H, Kim, B S, So, H S, Ryu, W S
Format: Article
Language:English
Subjects:
Adult
Aged
Female
Genotype
Hepacivirus - genetics
Hepacivirus - immunology
Hepatitis C - blood
Hepatitis C - diagnosis
Hepatitis C - drug therapy
Hepatitis C - immunology
Hepatitis C Antibodies - blood
Hepatitis C Antibodies - immunology
Hepatitis C Antigens - immunology
Humans
Immunoblotting
Interferon-alpha - therapeutic use
Male
Middle Aged
Recombinant Fusion Proteins - immunology
Recombinant Proteins
RNA, Viral - blood
Viral Core Proteins - immunology
Viral Nonstructural Proteins - immunology
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Summary:To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518), in which a domain of Core and NS3 of hepatitis C virus (HCV) was fused, ELISA was performed in a total of 76 serum samples. Each serum was serially diluted using two-fold dilution method with distilled water into 10 concentrations. They were all positive for second generation anti-HCV assay (HCV EIA II; Abbott Laboratories). Genotyping RT-PCR, quantitative competitive RT-PCR, and RIBA (Lucky Confirm; LG Biotech) were also assayed. Anti-Core 518 antibody was detected in x 12800 or higher dilutions of sera from 35 of 43 chronic hepatitis C (81.4%) and nine of 16 hepatocellular carcinoma sera (56.3%), one of four cirrhosis (25%), 0 of four acute hepatitis C, and one of nine healthy isolated anti-HCV-positive subjects (p=0.0000). The anti-Core 518 antibody titers were well correlated with the presence of HCV RNA in serum (p=0.002). The anti-Core 518 antibody titers decreased significantly in nine of ten responders to IFN-alpha treatment. Monitoring anti-Core 518 titers may be helpful not only for differentiating the status of HCV infection among patients with various type C viral liver diseases, but also for predicting responses to IFN-alpha treatment.
ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.1999.14.2.165