Exposure to toluene diisocyanate (TDI) induces IL-8 production from bronchial epithelial cells: effect of pro-inflammatory cytokines

This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compar...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Korean medical science 2003-12, Vol.18 (6), p.809-812
Main Authors: Lee, Young-Mok, Kim, Hyun-Ah, Park, Hae-Sim, Lee, Soo-Keol, Nahm, Dong-Ho
Format: Article
Language:English
Subjects:
Asthma - immunology
Bronchi - cytology
Bronchi - metabolism
Cell Line
Dexamethasone - pharmacology
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Glucocorticoids - pharmacology
Humans
Interleukin-8 - metabolism
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Toluene 2,4-Diisocyanate - toxicity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TNF alpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDI-induced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.
ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.2003.18.6.809