D‐TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo

We identify Drosophila TACC (D‐TACC) as a novel protein that is concentrated at centrosomes and interacts with microtubules. We show that D‐TACC is essential for normal spindle function in the early embryo; if D‐TACC function is perturbed by mutation or antibody injection, the microtubules emanating...

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Bibliographic Details
Published in:The EMBO journal 2000-01, Vol.19 (2), p.241-252
Main Authors: Gergely, Fanni, Kidd, Deborah, Jeffers, Kim, Wakefield, James G., Raff, Jordan W.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
cancer
centrosome
Centrosome - physiology
centrosomes
Chromosomes
Cloning, Molecular
Conserved Sequence
D-TACC protein
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Embryos
Gene Expression Regulation, Developmental
Humans
Mammals
Microtubule-Associated Proteins - chemistry
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
microtubules
mitosis
mitotic spindle
Molecular Sequence Data
Protein Structure, Secondary
Proteins
Recombinant Fusion Proteins - metabolism
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Spindle Apparatus - physiology
TACC protein
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Summary:We identify Drosophila TACC (D‐TACC) as a novel protein that is concentrated at centrosomes and interacts with microtubules. We show that D‐TACC is essential for normal spindle function in the early embryo; if D‐TACC function is perturbed by mutation or antibody injection, the microtubules emanating from centrosomes in embryos are short and chromosomes often fail to segregate properly. The C‐terminal region of D‐TACC interacts, possibly indirectly, with microtubules, and can target a heterologous fusion protein to centrosomes and microtubules in embryos. This C‐terminal region is related to the mammalian transforming, acidic, coiled‐coil‐containing (TACC) family of proteins. The function of the TACC proteins is unknown, but the genes encoding the known TACC proteins are all associated with genomic regions that are rearranged in certain cancers. We show that at least one of the mammalian TACC proteins appears to be associated with centrosomes and microtubules in human cells. We propose that this conserved C‐terminal ‘TACC domain’ defines a new family of microtubule‐interacting proteins.
ISSN:0261-4189
1460-2075
DOI:10.1093/emboj/19.2.241