Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement

Virus‐encoded movement protein (MP) mediates cell‐to‐cell spread of tobacco mosaic virus (TMV) through plant intercellular connections, the plasmodesmata. The molecular pathway by which TMV MP interacts with the host cell is largely unknown. To understand this process better, a cell wall‐associated...

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Published in:The EMBO journal 2000-03, Vol.19 (5), p.913-920
Main Authors: Chen, Min-Huei, Sheng, Jinsong, Hind, Geoffrey, Handa, Avtar K., Citovsky, Vitaly
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Binding Sites
Carboxylic Ester Hydrolases - metabolism
Cauliflower mosaic virus
Inactivation
Molecular Sequence Data
movement protein
movement proteins
MP protein
Nicotiana - metabolism
Nicotiana - virology
Nicotiana tabacum
pectin methylesterase
pectin methylesterases
Plant Viral Movement Proteins
plant viruses
Plants, Toxic
plasmodesmata
Protein Binding
Sequence Alignment
Tobacco
Tobacco mosaic virus
Tobacco Mosaic Virus - physiology
Turnip vein-clearing virus
Viral Proteins - metabolism
Virus Replication
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Citovsky, Vitaly
description Virus‐encoded movement protein (MP) mediates cell‐to‐cell spread of tobacco mosaic virus (TMV) through plant intercellular connections, the plasmodesmata. The molecular pathway by which TMV MP interacts with the host cell is largely unknown. To understand this process better, a cell wall‐associated protein that specifically binds the viral MP was purified from tobacco leaf cell walls and identified as pectin methylesterase (PME). In addition to TMV MP, PME is recognized by MPs of turnip vein clearing virus (TVCV) and cauliflower mosaic virus (CaMV). The use of amino acid deletion mutants of TMV MP showed that its domain was necessary and sufficient for association with PME. Deletion of the PME‐binding region resulted in inactivation of TMV cell‐to‐cell movement.
doi_str_mv 10.1093/emboj/19.5.913
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identifier ISSN: 0261-4189
ispartof The EMBO journal, 2000-03, Vol.19 (5), p.913-920
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language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_305631
source PubMed Central
subjects Amino Acid Sequence
Amino acids
Binding Sites
Carboxylic Ester Hydrolases - metabolism
Cauliflower mosaic virus
Inactivation
Molecular Sequence Data
movement protein
movement proteins
MP protein
Nicotiana - metabolism
Nicotiana - virology
Nicotiana tabacum
pectin methylesterase
pectin methylesterases
Plant Viral Movement Proteins
plant viruses
Plants, Toxic
plasmodesmata
Protein Binding
Sequence Alignment
Tobacco
Tobacco mosaic virus
Tobacco Mosaic Virus - physiology
Turnip vein-clearing virus
Viral Proteins - metabolism
Virus Replication
title Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement
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