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Transient micro-elastography:A novel non-invasive approach to measure liver stiffness in mice

AIM:To develop and validate a transient micro-elastography device to measure liver stiffness(LS) in mice.METHODS:A novel transient micro-elastography(TME) device,dedicated to LS measurements in mice with a range of measurement from 1-170 kPa,was developed using an optimized vibration frequency of 30...

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Published in:World journal of gastroenterology : WJG 2011-02, Vol.17 (8), p.968-975
Main Authors: Bastard, Cécile, Bosisio, Matteo R, Chabert, Michèle, Kalopissis, Athina D, Mahrouf-Yorgov, Meriem, Gilgenkrantz, Hélène, Mueller, Sebastian, Sandrin, Laurent
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Language:English
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Summary:AIM:To develop and validate a transient micro-elastography device to measure liver stiffness(LS) in mice.METHODS:A novel transient micro-elastography(TME) device,dedicated to LS measurements in mice with a range of measurement from 1-170 kPa,was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston.The novel probe was validated in a classical fibrosis model(CCl4) and in a transgenic murine model of systemic amyloidosis.RESULTS:TME could be successfully performed in control mice below the xiphoid cartilage,with a mean LS of 4.4 ± 1.3 kPa,a mean success rate of 88%,and an excellent intra-observer agreement(0.98).Treatment with CCl4 over seven weeks drastically increased LS as compared to controls(18.2 ± 3.7 kPa vs 3.6 ± 1.2 kPa).Moreover,fibrosis stage was highly correlated with LS(Spearman coefficient = 0.88,P 〈 0.01).In the amyloidosis model,much higher LS values were obtained,reaching maximum values of 〉 150 kPa.LS significantly correlated with the amyloidosis index(0.93,P 〈 0.0001) and the plasma concentration of mutant hapoA-□(0.62,P 〈 0.005).CONCLUSION:Here,we have established the first non-invasive approach to measure LS in mice,and have successfully validated it in two murine models of high LS.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v17.i8.968