Nucleotide sequence of the serine protease gene of Staphylococcus aureus, strain V8

The authors have cloned the serine protease gene of Staphylococcus aureus , strain V8, as a 2.1 kilobase HindIII fragment in pBR322. The primary structure, deduced from the nucleotide sequence, contains two notable features not revealed by the directly determined partial amino acid sequence. First,...

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Published in:Nucleic acids research 1987-08, Vol.15 (16), p.6757-6757
Main Authors: CARMONA, C, GRAY, G. L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
cloning
Endopeptidases - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetics
Microbiology
nucleotide sequence
Serine Endopeptidases
serine proteinase
Staphylococcus aureus
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
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GRAY, G. L
description The authors have cloned the serine protease gene of Staphylococcus aureus , strain V8, as a 2.1 kilobase HindIII fragment in pBR322. The primary structure, deduced from the nucleotide sequence, contains two notable features not revealed by the directly determined partial amino acid sequence. First, the primary translation product contains a 68 residue NH2-terminal sequence not present in the secreted form of the protease. This extension, which begins with a signal peptide of 19-29 residues, is analogous to the prepro regions of the alkaline proteases of Bacillus species. Second, the COOH-terminal region, whose unusual amino acid composition is known, contains a twelvefold repeated tripeptide (residues 221-256) having proline in the first position, asparagine or aspartic acid in the second position, and, with a single exception, asparagine in the third position.
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source PubMed Central; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects Amino Acid Sequence
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
cloning
Endopeptidases - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetics
Microbiology
nucleotide sequence
Serine Endopeptidases
serine proteinase
Staphylococcus aureus
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
title Nucleotide sequence of the serine protease gene of Staphylococcus aureus, strain V8
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