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Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome
MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNA...
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Published in: | European journal of human genetics : EJHG 2011-03, Vol.19 (3), p.313-319 |
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container_title | European journal of human genetics : EJHG |
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creator | Dostalova Merkerova, Michaela Krejcik, Zdenek Votavova, Hana Belickova, Monika Vasikova, Alzbeta Cermak, Jaroslav |
description | MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated (
n
=13) and downregulated (
n
=9) miRNAs were found (
P |
doi_str_mv | 10.1038/ejhg.2010.209 |
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n
=13) and downregulated (
n
=9) miRNAs were found (
P
<0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143
*
and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</description><identifier>ISSN: 1018-4813</identifier><identifier>EISSN: 1476-5438</identifier><identifier>DOI: 10.1038/ejhg.2010.209</identifier><identifier>PMID: 21150891</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>631/208/191/2018 ; 692/698/1543/233/1343 ; 692/699/1541 ; Acute myeloid leukemia ; Adult ; Aged ; Analysis of Variance ; Antigens, CD34 - analysis ; Antigens, CD34 - immunology ; Apoptosis ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Blast cells ; Blood transfusions ; Bone marrow ; Cancer ; CD34 antigen ; Chromosome 14 ; Chromosomes ; Clonal deletion ; Cytogenetics ; Down-Regulation ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression ; Gene Expression Profiling ; General aspects. Genetic counseling ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Hematologic and hematopoietic diseases ; Hematology ; Hematopoiesis ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - immunology ; Hemopoiesis ; Human Genetics ; Humans ; Leukemia ; Leukemia, Myeloid, Acute - metabolism ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical genetics ; Medical sciences ; Microarray Analysis ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; MiRNA ; Molecular and cellular biology ; Multigene Family ; Myelodysplastic syndrome ; Myelodysplastic Syndromes - genetics ; Myelodysplastic Syndromes - pathology ; Myeloid leukemia ; non-coding RNA ; Patients ; Refractory anemia ; Stem cells ; Up-Regulation ; Young Adult</subject><ispartof>European journal of human genetics : EJHG, 2011-03, Vol.19 (3), p.313-319</ispartof><rights>Macmillan Publishers Limited 2011</rights><rights>2015 INIST-CNRS</rights><rights>2011 Macmillan Publishers Limited All rights reserved 1018-4813/11</rights><rights>Copyright Nature Publishing Group Mar 2011</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2011 Macmillan Publishers Limited 2011 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</citedby><cites>FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061996/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061996/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23884507$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21150891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00598940$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Dostalova Merkerova, Michaela</creatorcontrib><creatorcontrib>Krejcik, Zdenek</creatorcontrib><creatorcontrib>Votavova, Hana</creatorcontrib><creatorcontrib>Belickova, Monika</creatorcontrib><creatorcontrib>Vasikova, Alzbeta</creatorcontrib><creatorcontrib>Cermak, Jaroslav</creatorcontrib><title>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</title><title>European journal of human genetics : EJHG</title><addtitle>Eur J Hum Genet</addtitle><addtitle>Eur J Hum Genet</addtitle><description>MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated (
n
=13) and downregulated (
n
=9) miRNAs were found (
P
<0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143
*
and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</description><subject>631/208/191/2018</subject><subject>692/698/1543/233/1343</subject><subject>692/699/1541</subject><subject>Acute myeloid leukemia</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis of Variance</subject><subject>Antigens, CD34 - analysis</subject><subject>Antigens, CD34 - immunology</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blast cells</subject><subject>Blood transfusions</subject><subject>Bone marrow</subject><subject>Cancer</subject><subject>CD34 antigen</subject><subject>Chromosome 14</subject><subject>Chromosomes</subject><subject>Clonal deletion</subject><subject>Cytogenetics</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>General aspects. Genetic counseling</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Hematopoiesis</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Hemopoiesis</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia, Myeloid, Acute - metabolism</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Microarray Analysis</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>MiRNA</subject><subject>Molecular and cellular biology</subject><subject>Multigene Family</subject><subject>Myelodysplastic syndrome</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>Myelodysplastic Syndromes - pathology</subject><subject>Myeloid leukemia</subject><subject>non-coding RNA</subject><subject>Patients</subject><subject>Refractory anemia</subject><subject>Stem cells</subject><subject>Up-Regulation</subject><subject>Young Adult</subject><issn>1018-4813</issn><issn>1476-5438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kV2LEzEUhgdR3HX10luJgojI1JPJ941QuuoKRUH0OqQzmTZlmswm0-7232-G1q4ueJOv85z3nJy3KF5imGAg8qNdr5aTCvK1AvWoOMdU8JJRIh_nM2BZUonJWfEspTVADgr8tDirMGYgFT4vwqVLg_P14HYWbVwdw8_vU2Rv-2hTcsGjPobWdTYh59HsktAPaBF8Rk2M4QbVtusSamPYoN4MzvohoRs3rNBmb7vQ7FPfmaxfo7T3Tabs8-JJa7pkXxz3i-L3l8-_Zlfl_MfXb7PpvKyZoEPJQTAiLAfcKJC2lkRiA4K3DW8Na6StGFSYSdq2CwxcMqskpZbIinNiBCcXxaeDbr9dbGxT586i6XQfXe58r4Nx-t-Idyu9DDtNgGOlRoH3B4HVg7Sr6VyPbwBMSUVhhzP77lgshuutTYPeuDSOxngbtklLLkRuTUEm3zwg12EbfZ6ElqwiVAkpMlQeoOxGStG2p_oY9Gi6Hk3Xo-l5UZl_9fdfT_QflzPw9giYVJuujcbXLt1zRErKYCw8OXAph_zSxvvu_lf59SHBm2Eb7UlxpEZoZO4AA_TPuw</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Dostalova Merkerova, Michaela</creator><creator>Krejcik, Zdenek</creator><creator>Votavova, Hana</creator><creator>Belickova, Monika</creator><creator>Vasikova, Alzbeta</creator><creator>Cermak, Jaroslav</creator><general>Springer International Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7TM</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</title><author>Dostalova Merkerova, Michaela ; Krejcik, Zdenek ; Votavova, Hana ; Belickova, Monika ; Vasikova, Alzbeta ; Cermak, Jaroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>631/208/191/2018</topic><topic>692/698/1543/233/1343</topic><topic>692/699/1541</topic><topic>Acute myeloid leukemia</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis of Variance</topic><topic>Antigens, CD34 - analysis</topic><topic>Antigens, CD34 - immunology</topic><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blast cells</topic><topic>Blood transfusions</topic><topic>Bone marrow</topic><topic>Cancer</topic><topic>CD34 antigen</topic><topic>Chromosome 14</topic><topic>Chromosomes</topic><topic>Clonal deletion</topic><topic>Cytogenetics</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>General aspects. Genetic counseling</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Hematopoiesis</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Hemopoiesis</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia, Myeloid, Acute - metabolism</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Microarray Analysis</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>MiRNA</topic><topic>Molecular and cellular biology</topic><topic>Multigene Family</topic><topic>Myelodysplastic syndrome</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>Myelodysplastic Syndromes - pathology</topic><topic>Myeloid leukemia</topic><topic>non-coding RNA</topic><topic>Patients</topic><topic>Refractory anemia</topic><topic>Stem cells</topic><topic>Up-Regulation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dostalova Merkerova, Michaela</creatorcontrib><creatorcontrib>Krejcik, Zdenek</creatorcontrib><creatorcontrib>Votavova, Hana</creatorcontrib><creatorcontrib>Belickova, Monika</creatorcontrib><creatorcontrib>Vasikova, Alzbeta</creatorcontrib><creatorcontrib>Cermak, Jaroslav</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European journal of human genetics : EJHG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dostalova Merkerova, Michaela</au><au>Krejcik, Zdenek</au><au>Votavova, Hana</au><au>Belickova, Monika</au><au>Vasikova, Alzbeta</au><au>Cermak, Jaroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</atitle><jtitle>European journal of human genetics : EJHG</jtitle><stitle>Eur J Hum Genet</stitle><addtitle>Eur J Hum Genet</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>19</volume><issue>3</issue><spage>313</spage><epage>319</epage><pages>313-319</pages><issn>1018-4813</issn><eissn>1476-5438</eissn><abstract>MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated (
n
=13) and downregulated (
n
=9) miRNAs were found (
P
<0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143
*
and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>21150891</pmid><doi>10.1038/ejhg.2010.209</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 631/208/191/2018 692/698/1543/233/1343 692/699/1541 Acute myeloid leukemia Adult Aged Analysis of Variance Antigens, CD34 - analysis Antigens, CD34 - immunology Apoptosis Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedicine Blast cells Blood transfusions Bone marrow Cancer CD34 antigen Chromosome 14 Chromosomes Clonal deletion Cytogenetics Down-Regulation Female Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Gene Expression Profiling General aspects. Genetic counseling Genetics Genetics of eukaryotes. Biological and molecular evolution Hematologic and hematopoietic diseases Hematology Hematopoiesis Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - immunology Hemopoiesis Human Genetics Humans Leukemia Leukemia, Myeloid, Acute - metabolism Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical genetics Medical sciences Microarray Analysis MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism Middle Aged MiRNA Molecular and cellular biology Multigene Family Myelodysplastic syndrome Myelodysplastic Syndromes - genetics Myelodysplastic Syndromes - pathology Myeloid leukemia non-coding RNA Patients Refractory anemia Stem cells Up-Regulation Young Adult |
title | Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome |
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