Loading…

Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome

MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNA...

Full description

Saved in:
Bibliographic Details
Published in:European journal of human genetics : EJHG 2011-03, Vol.19 (3), p.313-319
Main Authors: Dostalova Merkerova, Michaela, Krejcik, Zdenek, Votavova, Hana, Belickova, Monika, Vasikova, Alzbeta, Cermak, Jaroslav
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763
cites cdi_FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763
container_end_page 319
container_issue 3
container_start_page 313
container_title European journal of human genetics : EJHG
container_volume 19
creator Dostalova Merkerova, Michaela
Krejcik, Zdenek
Votavova, Hana
Belickova, Monika
Vasikova, Alzbeta
Cermak, Jaroslav
description MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated ( n =13) and downregulated ( n =9) miRNAs were found ( P
doi_str_mv 10.1038/ejhg.2010.209
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3061996</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2268862781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</originalsourceid><addsrcrecordid>eNp1kV2LEzEUhgdR3HX10luJgojI1JPJ941QuuoKRUH0OqQzmTZlmswm0-7232-G1q4ueJOv85z3nJy3KF5imGAg8qNdr5aTCvK1AvWoOMdU8JJRIh_nM2BZUonJWfEspTVADgr8tDirMGYgFT4vwqVLg_P14HYWbVwdw8_vU2Rv-2hTcsGjPobWdTYh59HsktAPaBF8Rk2M4QbVtusSamPYoN4MzvohoRs3rNBmb7vQ7FPfmaxfo7T3Tabs8-JJa7pkXxz3i-L3l8-_Zlfl_MfXb7PpvKyZoEPJQTAiLAfcKJC2lkRiA4K3DW8Na6StGFSYSdq2CwxcMqskpZbIinNiBCcXxaeDbr9dbGxT586i6XQfXe58r4Nx-t-Idyu9DDtNgGOlRoH3B4HVg7Sr6VyPbwBMSUVhhzP77lgshuutTYPeuDSOxngbtklLLkRuTUEm3zwg12EbfZ6ElqwiVAkpMlQeoOxGStG2p_oY9Gi6Hk3Xo-l5UZl_9fdfT_QflzPw9giYVJuujcbXLt1zRErKYCw8OXAph_zSxvvu_lf59SHBm2Eb7UlxpEZoZO4AA_TPuw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>852349787</pqid></control><display><type>article</type><title>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</title><source>PubMed (Medline)</source><creator>Dostalova Merkerova, Michaela ; Krejcik, Zdenek ; Votavova, Hana ; Belickova, Monika ; Vasikova, Alzbeta ; Cermak, Jaroslav</creator><creatorcontrib>Dostalova Merkerova, Michaela ; Krejcik, Zdenek ; Votavova, Hana ; Belickova, Monika ; Vasikova, Alzbeta ; Cermak, Jaroslav</creatorcontrib><description>MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated ( n =13) and downregulated ( n =9) miRNAs were found ( P &lt;0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143 * and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</description><identifier>ISSN: 1018-4813</identifier><identifier>EISSN: 1476-5438</identifier><identifier>DOI: 10.1038/ejhg.2010.209</identifier><identifier>PMID: 21150891</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>631/208/191/2018 ; 692/698/1543/233/1343 ; 692/699/1541 ; Acute myeloid leukemia ; Adult ; Aged ; Analysis of Variance ; Antigens, CD34 - analysis ; Antigens, CD34 - immunology ; Apoptosis ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Blast cells ; Blood transfusions ; Bone marrow ; Cancer ; CD34 antigen ; Chromosome 14 ; Chromosomes ; Clonal deletion ; Cytogenetics ; Down-Regulation ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression ; Gene Expression Profiling ; General aspects. Genetic counseling ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Hematologic and hematopoietic diseases ; Hematology ; Hematopoiesis ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - immunology ; Hemopoiesis ; Human Genetics ; Humans ; Leukemia ; Leukemia, Myeloid, Acute - metabolism ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical genetics ; Medical sciences ; Microarray Analysis ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; MiRNA ; Molecular and cellular biology ; Multigene Family ; Myelodysplastic syndrome ; Myelodysplastic Syndromes - genetics ; Myelodysplastic Syndromes - pathology ; Myeloid leukemia ; non-coding RNA ; Patients ; Refractory anemia ; Stem cells ; Up-Regulation ; Young Adult</subject><ispartof>European journal of human genetics : EJHG, 2011-03, Vol.19 (3), p.313-319</ispartof><rights>Macmillan Publishers Limited 2011</rights><rights>2015 INIST-CNRS</rights><rights>2011 Macmillan Publishers Limited All rights reserved 1018-4813/11</rights><rights>Copyright Nature Publishing Group Mar 2011</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2011 Macmillan Publishers Limited 2011 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</citedby><cites>FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061996/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061996/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=23884507$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21150891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00598940$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Dostalova Merkerova, Michaela</creatorcontrib><creatorcontrib>Krejcik, Zdenek</creatorcontrib><creatorcontrib>Votavova, Hana</creatorcontrib><creatorcontrib>Belickova, Monika</creatorcontrib><creatorcontrib>Vasikova, Alzbeta</creatorcontrib><creatorcontrib>Cermak, Jaroslav</creatorcontrib><title>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</title><title>European journal of human genetics : EJHG</title><addtitle>Eur J Hum Genet</addtitle><addtitle>Eur J Hum Genet</addtitle><description>MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated ( n =13) and downregulated ( n =9) miRNAs were found ( P &lt;0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143 * and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</description><subject>631/208/191/2018</subject><subject>692/698/1543/233/1343</subject><subject>692/699/1541</subject><subject>Acute myeloid leukemia</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis of Variance</subject><subject>Antigens, CD34 - analysis</subject><subject>Antigens, CD34 - immunology</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blast cells</subject><subject>Blood transfusions</subject><subject>Bone marrow</subject><subject>Cancer</subject><subject>CD34 antigen</subject><subject>Chromosome 14</subject><subject>Chromosomes</subject><subject>Clonal deletion</subject><subject>Cytogenetics</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>General aspects. Genetic counseling</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Hematopoiesis</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Hemopoiesis</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia, Myeloid, Acute - metabolism</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Microarray Analysis</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>MiRNA</subject><subject>Molecular and cellular biology</subject><subject>Multigene Family</subject><subject>Myelodysplastic syndrome</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>Myelodysplastic Syndromes - pathology</subject><subject>Myeloid leukemia</subject><subject>non-coding RNA</subject><subject>Patients</subject><subject>Refractory anemia</subject><subject>Stem cells</subject><subject>Up-Regulation</subject><subject>Young Adult</subject><issn>1018-4813</issn><issn>1476-5438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kV2LEzEUhgdR3HX10luJgojI1JPJ941QuuoKRUH0OqQzmTZlmswm0-7232-G1q4ueJOv85z3nJy3KF5imGAg8qNdr5aTCvK1AvWoOMdU8JJRIh_nM2BZUonJWfEspTVADgr8tDirMGYgFT4vwqVLg_P14HYWbVwdw8_vU2Rv-2hTcsGjPobWdTYh59HsktAPaBF8Rk2M4QbVtusSamPYoN4MzvohoRs3rNBmb7vQ7FPfmaxfo7T3Tabs8-JJa7pkXxz3i-L3l8-_Zlfl_MfXb7PpvKyZoEPJQTAiLAfcKJC2lkRiA4K3DW8Na6StGFSYSdq2CwxcMqskpZbIinNiBCcXxaeDbr9dbGxT586i6XQfXe58r4Nx-t-Idyu9DDtNgGOlRoH3B4HVg7Sr6VyPbwBMSUVhhzP77lgshuutTYPeuDSOxngbtklLLkRuTUEm3zwg12EbfZ6ElqwiVAkpMlQeoOxGStG2p_oY9Gi6Hk3Xo-l5UZl_9fdfT_QflzPw9giYVJuujcbXLt1zRErKYCw8OXAph_zSxvvu_lf59SHBm2Eb7UlxpEZoZO4AA_TPuw</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Dostalova Merkerova, Michaela</creator><creator>Krejcik, Zdenek</creator><creator>Votavova, Hana</creator><creator>Belickova, Monika</creator><creator>Vasikova, Alzbeta</creator><creator>Cermak, Jaroslav</creator><general>Springer International Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7TM</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</title><author>Dostalova Merkerova, Michaela ; Krejcik, Zdenek ; Votavova, Hana ; Belickova, Monika ; Vasikova, Alzbeta ; Cermak, Jaroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>631/208/191/2018</topic><topic>692/698/1543/233/1343</topic><topic>692/699/1541</topic><topic>Acute myeloid leukemia</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis of Variance</topic><topic>Antigens, CD34 - analysis</topic><topic>Antigens, CD34 - immunology</topic><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blast cells</topic><topic>Blood transfusions</topic><topic>Bone marrow</topic><topic>Cancer</topic><topic>CD34 antigen</topic><topic>Chromosome 14</topic><topic>Chromosomes</topic><topic>Clonal deletion</topic><topic>Cytogenetics</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>General aspects. Genetic counseling</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Hematopoiesis</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Hemopoiesis</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia, Myeloid, Acute - metabolism</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Microarray Analysis</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>MiRNA</topic><topic>Molecular and cellular biology</topic><topic>Multigene Family</topic><topic>Myelodysplastic syndrome</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>Myelodysplastic Syndromes - pathology</topic><topic>Myeloid leukemia</topic><topic>non-coding RNA</topic><topic>Patients</topic><topic>Refractory anemia</topic><topic>Stem cells</topic><topic>Up-Regulation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dostalova Merkerova, Michaela</creatorcontrib><creatorcontrib>Krejcik, Zdenek</creatorcontrib><creatorcontrib>Votavova, Hana</creatorcontrib><creatorcontrib>Belickova, Monika</creatorcontrib><creatorcontrib>Vasikova, Alzbeta</creatorcontrib><creatorcontrib>Cermak, Jaroslav</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European journal of human genetics : EJHG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dostalova Merkerova, Michaela</au><au>Krejcik, Zdenek</au><au>Votavova, Hana</au><au>Belickova, Monika</au><au>Vasikova, Alzbeta</au><au>Cermak, Jaroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome</atitle><jtitle>European journal of human genetics : EJHG</jtitle><stitle>Eur J Hum Genet</stitle><addtitle>Eur J Hum Genet</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>19</volume><issue>3</issue><spage>313</spage><epage>319</epage><pages>313-319</pages><issn>1018-4813</issn><eissn>1476-5438</eissn><abstract>MicroRNAs (miRNAs) are small non-coding RNAs functioning as regulators of hematopoiesis. Their differential expression patterns have been linked with various pathological processes originating from hematopoietic stem cells (HSCs). However, limited information is available regarding the role of miRNAs in myelodysplastic syndrome (MDS). Using miRNA arrays, we measured expression of 1,145 miRNAs in CD34+ bone marrow cells obtained from 39 MDS and acute myeloid leukemia (AML) evolved from MDS patients, and compared them with those of six healthy donors. Differential miRNA expression was analyzed and a panel of upregulated ( n =13) and downregulated ( n =9) miRNAs were found ( P &lt;0.001) in MDS/AML patients. An increased expression of a large miRNA cluster mapped within the 14q32 locus was detected. Differences in miRNA expression of MDS subtypes showed a distinction between early and advanced MDS; an apparent dissimilarity was observed between RAEB-1 and RAEB-2 subtypes. In early MDS, we monitored upregulation of proapoptotic miR-34a, which may contribute to the increased apoptosis of HSCs. Patients with 5q deletion were characterized by decreased levels of miR-143 * and miR-378 mapped within the commonly deleted region at 5q32. This is an early report describing differential expression in MDS CD34+ cells, likely reflecting their disease-specific regulation.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>21150891</pmid><doi>10.1038/ejhg.2010.209</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1018-4813
ispartof European journal of human genetics : EJHG, 2011-03, Vol.19 (3), p.313-319
issn 1018-4813
1476-5438
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3061996
source PubMed (Medline)
subjects 631/208/191/2018
692/698/1543/233/1343
692/699/1541
Acute myeloid leukemia
Adult
Aged
Analysis of Variance
Antigens, CD34 - analysis
Antigens, CD34 - immunology
Apoptosis
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Blast cells
Blood transfusions
Bone marrow
Cancer
CD34 antigen
Chromosome 14
Chromosomes
Clonal deletion
Cytogenetics
Down-Regulation
Female
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene Expression
Gene Expression Profiling
General aspects. Genetic counseling
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Hematologic and hematopoietic diseases
Hematology
Hematopoiesis
Hematopoietic stem cells
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - immunology
Hemopoiesis
Human Genetics
Humans
Leukemia
Leukemia, Myeloid, Acute - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical genetics
Medical sciences
Microarray Analysis
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
MiRNA
Molecular and cellular biology
Multigene Family
Myelodysplastic syndrome
Myelodysplastic Syndromes - genetics
Myelodysplastic Syndromes - pathology
Myeloid leukemia
non-coding RNA
Patients
Refractory anemia
Stem cells
Up-Regulation
Young Adult
title Distinctive microRNA expression profiles in CD34+ bone marrow cells from patients with myelodysplastic syndrome
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T19%3A04%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distinctive%20microRNA%20expression%20profiles%20in%20CD34+%20bone%20marrow%20cells%20from%20patients%20with%20myelodysplastic%20syndrome&rft.jtitle=European%20journal%20of%20human%20genetics%20:%20EJHG&rft.au=Dostalova%20Merkerova,%20Michaela&rft.date=2011-03-01&rft.volume=19&rft.issue=3&rft.spage=313&rft.epage=319&rft.pages=313-319&rft.issn=1018-4813&rft.eissn=1476-5438&rft_id=info:doi/10.1038/ejhg.2010.209&rft_dat=%3Cproquest_pubme%3E2268862781%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c574t-607537e601d908ec8381a076fd6fa5d8e25021584ffb10685e9844e382663a763%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=852349787&rft_id=info:pmid/21150891&rfr_iscdi=true