Localization of AQP5 during development of the mouse submandibular salivary gland

Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution of AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues to its unknown role during development. In the present work, SMGs from embryonic da...

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Bibliographic Details
Published in:Journal of molecular histology 2011-02, Vol.42 (1), p.71-81
Main Authors: Larsen, Helga S, Aure, Marit H, Peters, Sarah B, Larsen, Melinda, Messelt, Edward B, Kanli Galtung, Hilde
Format: Article
Language:English
Subjects:
Animals
AQP5
Aquaporin 5 - metabolism
Biomedical and Life Sciences
Biomedicine
Cell Biology
Development
Developmental Biology
Female
Gene Expression Regulation, Developmental
Intracellular Space - metabolism
Life Sciences
Male
Mice
Original Paper
Postnatal
Prenatal
Protein Transport
sexual dimorphism
Submandibular gland
Submandibular Gland - cytology
Submandibular Gland - embryology
Submandibular Gland - growth & development
Submandibular Gland - ultrastructure
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Summary:Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution of AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues to its unknown role during development. In the present work, SMGs from embryonic day (E) 14.5-18.5 and postnatal days (P) 0, 2, 5, 25, and 60 were immunostained for AQP5 and analyzed using light microscopy. Additional confocal and transmission electron microscopy were performed on P60 glands. Our results show that AQP5 expression first occurs in a scattered pattern in the late canalicular stage and becomes more prominent and organized in the terminal tubuli/pro-acinar cells towards birth. Additional apical membrane staining in the entire intralobular duct is found just prior to birth. During postnatal development, AQP5 is expressed in both the luminal and lateral membrane of pro-acinar/acinar cells. AQP5 is also detected in the basal membrane of acinar cells at P25 and P60. In the intercalated ducts at P60, the male glands show apical staining in the entire segment, while only the proximal region is positive in the female glands. These results demonstrate an evolving distribution of AQP5 during pre- and postnatal development in the mouse SMGs.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-010-9308-0