Transcription of the tRNA-tufB operon of Escherichia coli: activation, termination and antitermination

Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90...

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Bibliographic Details
Published in:Nucleic acids research 1987-11, Vol.15 (22), p.9515-9530
Main Authors: VAN DELFT, J. H. M, MARINON, B, SCHMIDT, D. S, BOSCH, L
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chromosome Deletion
Codon
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Galactokinase - genetics
Genes
Genes, Bacterial
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Operon
Plasmids
RNA, Transfer - genetics
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB transcription. This demonstrates the presence of a region, upstream of the tRNA-tufB promoter, that enhances the expression of the operon. DNA fragments bearing this upstream activator region do not display an abnormal electrophoretic mobility, as has been observed for the rrnB P1 upstream activator. Deletions starting in the first tRNA gene and directing towards tufB reveal at least two sites that influence tufB transcription. One signals transcription termination in the intergenic region between thrT and tufB. The other may be involved in antitermination. Possible mechanisms underlying antitermination and termination are considered in the light of the nucleotide sequence.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.22.9515