Linking of the human immunoglobulin VK and JKCK regions by chromosomal walking

The linking of the human VK and JKCK gene regions (abbreviations in ref. 1) by chromosomal walking is reported. Hybridization experiments with the DNA of a somatic cell hybrid containing the region between JKCK and the telomer show that none of the major VK gene clusters is located downstream of CK....

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Bibliographic Details
Published in:Nucleic acids research 1987-12, Vol.15 (23), p.9655-9665
Main Authors: KLOBECK, H.-G, ZIMMER, F.-J, COMBRIATO, G, ZACHAU, H. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome Mapping - methods
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genes, Immunoglobulin
Genes. Genome
Genetic Linkage
Humans
Immunoglobulin Constant Regions - genetics
Immunoglobulin Joining Region - genetics
Immunoglobulin kappa-Chains - genetics
Immunoglobulin Variable Region - genetics
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Recombination, Genetic
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Summary:The linking of the human VK and JKCK gene regions (abbreviations in ref. 1) by chromosomal walking is reported. Hybridization experiments with the DNA of a somatic cell hybrid containing the region between JKCK and the telomer show that none of the major VK gene clusters is located downstream of CK. The distance between the VK and JK genes was found to be 23 kb. The JK proximal VK gene is the B3 gene which is the only representative of subgroup IV in the genome. This gene and the neighbouring B2 gene (accompanying paper) are arranged in opposite orientation to JKCK and can therefore rearrange only by an inversion mechanism. This finding is used, together with previous data, to delineate the rearrangement processes in the Burkitt lymphoma derived cell line BL21 as comprising an inversion in the first and a deletion in the second step.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.23.9655