Determination of the origin cleavage and joining domain of geminivirus Rep proteins

Replication of the single-stranded DNA genome of plant geminiviruses follows a rolling circle mechanism. It strictly depends on a 'rolling circle replication initiator protein', the Mr 41 kDa viral Rep protein, encoded by the C1 or AC1 genes. Using wheat dwarf virus (WDV) and tomato yellow...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1995-03, Vol.23 (6), p.910-916
Main Authors: Heyraud-Nitschke, F, Schumacher, S, Laufs, J, Schaefer, S, Schell, J, Gronenborn, B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
DNA replication
DNA Replication - physiology
DNA, Single-Stranded - metabolism
Geminiviridae
Geminiviridae - physiology
Geminivirus
Genes, Viral - genetics
genome
Lycopersicon esculentum - virology
Molecular Sequence Data
Open Reading Frames - genetics
Polydeoxyribonucleotides - metabolism
Recombinant Fusion Proteins - biosynthesis
Replication Origin - genetics
Tomato yellow leaf curl virus
Triticum - virology
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Replication - physiology
Wheat dwarf virus
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Replication of the single-stranded DNA genome of plant geminiviruses follows a rolling circle mechanism. It strictly depends on a 'rolling circle replication initiator protein', the Mr 41 kDa viral Rep protein, encoded by the C1 or AC1 genes. Using wheat dwarf virus (WDV) and tomato yellow leaf curl virus (TYLCV) as examples, we show that not only the full-size Rep proteins, but also a putative 30 kDa translation product of WDV open reading frame C1-N as well as an artificially shortened 24 kDa Rep of TYLCV, cleave and join single-stranded origin DNA in vitro. Thus the pivotal origin recognition and processing activities of geminivirus Rep proteins must be mediated by the amino-terminal domain of Rep.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/23.6.910