8-Methoxypsoralen photoinduced plasmid-chromosome recombination in Saccharomyces cerevisiae using a centromeric vector

The characterization of a new system to study the induction of plasmid-chromosome recombination is described. Single-stranded and double-stranded centromeric vectors bearing 8-methoxypsoralen photoinduced lesions were used to transform a wild-type yeast strain bearing the leu2-3,112 marker. Using th...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1995-05, Vol.23 (9), p.1614-1620
Main Authors: Meira, Lisiane B., Pêgas Henriques, Joao Antonio, Magaña-Schwencke, Nieve
Format: Article
Language:English
Subjects:
Base Sequence
Centromere - genetics
Chromosomes - genetics
Chromosomes - metabolism
DNA Damage
DNA Repair - genetics
Genetic Vectors
Methoxsalen - metabolism
Molecular Sequence Data
Plasmids - genetics
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The characterization of a new system to study the induction of plasmid-chromosome recombination is described. Single-stranded and double-stranded centromeric vectors bearing 8-methoxypsoralen photoinduced lesions were used to transform a wild-type yeast strain bearing the leu2-3,112 marker. Using the SSCP methodology and DNA sequencing, it was demonstrated that repair of the lesions in plasmid DNA was mainly due to conversion of the chromosomal allele to the plasmid DNA.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/23.9.1614