Proteomic analysis of pancreatic intraepithelial neoplasia and pancreatic carcinoma in rat models

• Published in: World journal of gastroenterology : WJG 2011-03, Vol.17 (11), p.1434-1441
• Main Authors: Wang, Lei, Liu, Hai-Lin, Li, Ya, Yuan, Ping
• Format: Article
• Language: English
• Subjects: 9,10-Dimethyl-1,2-benzanthracene; Animals; Biomarkers, Tumor - metabolism; Carcinoma - chemically induced; Carcinoma - metabolism; Carcinoma - pathology; Carcinoma in Situ - chemically induced; Carcinoma in Situ - metabolism; Carcinoma in Situ - pathology; Chi-Square Distribution; Electrophoresis, Gel, Two-Dimensional; Immunohistochemistry; Male; Neoplasm Proteins - metabolism; Original; Pancreatic Neoplasms - chemically induced; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Phosphopyruvate Hydratase - metabolism; Proteomics - methods; Rats; Rats, Sprague-Dawley; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry; Time Factors; Tumor Protein, Translationally-Controlled 1; 乙酰基转移酶; 基质辅助激光解吸; 大鼠模型; 学分; 胰腺癌; 胰腺组织; 腺上皮; 蛋白质组
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v17.i11.1434

AIM：To detect the proteomic variabilities of pancreatic intraepithelial neoplasia（PanIN）and pancreatic carcinoma（PC）induced by 7,12-dimethylbenzanthracene（DMBA） in rat models and to identify potential biomarkers.METHODS：Sixty adult male Sprague Dawley rats were randomized into three groups.The rats had DMBA implanted into their pancreas for one（n=20）or two months（n=20）or assigned to the normal group（n =20）.The rats were killed after one or two months,and were evaluated histopathologically.Three tissue samples from each group of rats with either normal pancreas,PanIN（PanIN-2）or PC were examined by 2D-DIGE.The different expression spot features were analyzed by matrix-assisted laser desorption/ionizationtime of flight/time of flight（MALDI-TOF/TOF）tandem mass spectrometry.The expression of enolase 1,a differentially expressed protein,was identified by immu-nohistochemistry.RESULTS：There was significant difference in the proportions of neoplastic changes between the 1-and 2-mogroups（P=0.0488）.There was an increase in the frequency of adenocarcinomas in the 2-mo group compared with the 1-mo group（P=0.0309）.No neoplastic changes were observed in any of the animals in the normal group.Enolase 1,pancreatic ELA3B,necdin,Hbp23,CHD3,hnRNP A2/B1,Rap80,and Gnb2l1 were up-regulated in the PanIN and PC tissues,and CEL,TPT1,NME2,PCK2,an unnamed protein product,and glycine C-acetyltransferase were down-regulated in the PanIN and PC tissues.The immunohistochemical results showed that enolase 1 expression was up-regulated in the pancreatic cancer tissues of rats and humans.CONCLUSION：The pancreatic protein expression changes induced by DMBA suggest potential molecular targets for the early diagnosis and treatment of PC.