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Proteome of Human Subcutaneous Adipose Tissue Stromal Vascular Fraction Cells vs. Mature Adipocytes Based on DIGE

Adipose tissue contains a heterogeneous population of mature adipocytes, endothelial cells, immune cells, pericytes, and pre-adipocytic stromal/stem cells. To date, the majority of proteomic analyses have focused on intact adipose tissue or isolated adipose stromal/stem cells in vitro . In this stud...

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Bibliographic Details
Published in:Journal of proteome research 2011-03, Vol.10 (4), p.1519-1527
Main Authors: Kheterpal, Indu, Ku, Ginger, Coleman, Liana, Yu, Gang, Ptitsyn, Andrey A., Floyd, Z. Elizabeth, Gimble, Jeffrey M.
Format: Article
Language:English
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Summary:Adipose tissue contains a heterogeneous population of mature adipocytes, endothelial cells, immune cells, pericytes, and pre-adipocytic stromal/stem cells. To date, the majority of proteomic analyses have focused on intact adipose tissue or isolated adipose stromal/stem cells in vitro . In this study, human subcutaneous adipose tissue from multiple depots (arm and abdomen) obtained from female donors was separated into populations of stromal vascular fraction cells and mature adipocytes. Out of 960 features detected by 2-D gel electrophoresis, a total of 200 features displayed a 2-fold up- or down-regulation relative to each cell population. The protein identity of 136 features was determined. Immunoblot analyses comparing SVF relative to adipocytes confirmed that carbonic anhydrase II was up-regulated in both adipose depots while catalase was up-regulated in the arm only. Bioinformatic analyses of the dataset determined that cytoskeletal, glycogenic, glycolytic, lipid metabolic, and oxidative stress related pathways were highly represented as differentially regulated between the mature adipocytes and stromal vascular fraction cells. These findings extend previous reports in the literature with respect to the adipose tissue proteome and the consequences of adipogenesis. The proteins identified may have value as biomarkers for monitoring the physiology and pathology of cell populations within subcutaneous adipose depots.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr100887r