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A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans
Aims/hypothesis We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. Methods We studied pancreases obtained at autopsy from individuals ( n = 11) who had prior opposite-sex cord blood transplant...
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Published in: | Diabetologia 2011-05, Vol.54 (5), p.1066-1074 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Aims/hypothesis
We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans.
Methods
We studied pancreases obtained at autopsy from individuals (
n
= 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls (
n
= 11).
Results
In the men with prior transplant of female cord blood, there were 3.4 ± 0.3% XX-positive insulin-expressing islet cells compared with 0.32 ± 0.05% (
p
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ISSN: | 0012-186X 1432-0428 |
DOI: | 10.1007/s00125-011-2071-2 |