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A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans

Aims/hypothesis We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. Methods We studied pancreases obtained at autopsy from individuals ( n  = 11) who had prior opposite-sex cord blood transplant...

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Bibliographic Details
Published in:Diabetologia 2011-05, Vol.54 (5), p.1066-1074
Main Authors: Huang, C. J., Butler, A. E., Moran, A., Rao, P. N., Wagner, J. E., Blazar, B. R., Rizza, R. A., Manivel, J. C., Butler, P. C.
Format: Article
Language:English
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Summary:Aims/hypothesis We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. Methods We studied pancreases obtained at autopsy from individuals ( n  = 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls ( n  = 11). Results In the men with prior transplant of female cord blood, there were 3.4 ± 0.3% XX-positive insulin-expressing islet cells compared with 0.32 ± 0.05% ( p  
ISSN:0012-186X
1432-0428
DOI:10.1007/s00125-011-2071-2