Dynamic smad‐mediated BMP signaling revealed through transgenic zebrafish

Bone morphogenic protein (BMP) signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad‐mediated BMP signaling in embryos and adults. These lines express either enhanced green fluorescent protein (eGFP), destabilized eGFP, or...

Full description

Saved in:
Bibliographic Details
Published in:Developmental dynamics 2011-03, Vol.240 (3), p.712-722
Main Authors: Collery, Ross F., Link, Brian A.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
BMP response element
Bone Morphogenetic Proteins - metabolism
fluorescent reporter
Immunohistochemistry
in vivo imaging
Microscopy, Fluorescence
morphogen gradient
organogenesis
Response Elements - genetics
Response Elements - physiology
Signal Transduction - genetics
Signal Transduction - physiology
Smad Proteins - metabolism
transgenic zebrafish
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bone morphogenic protein (BMP) signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad‐mediated BMP signaling in embryos and adults. These lines express either enhanced green fluorescent protein (eGFP), destabilized eGFP, or destabilized Kusabira Orange 2 (KO2) under the well‐characterized BMP Response Element (BRE). These fluorescent proteins were found to be expressed dynamically in regions of known BMP signaling including the developing tail bud, hematopoietic lineage, dorsal eye, brain structures, heart, jaw, fins, and somites, as well as other tissues. Responsiveness to changes in BMP signaling was confirmed by observing fluorescence after activation in an hsp70:bmp2b transgenic background or by inhibition in an hsp70:nog3 background. We further demonstrated faithful reportage by the BRE transgenic lines following chemical repression of BMP signaling using an inhibitor of BMP receptor activity, dorsomorphin. Overall, these lines will serve as valuable tools to explore the mechanisms and regulation of BMP signal during embryogenesis, in tissue maintenance, and during disease. Developmental Dynamics 240:712–722, 2011. © 2011 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22567