Functional connectome of the striatal medium spiny neuron

Dopamine system disorders ranging from movement disorders to addiction and schizophrenia involve striatal medium spiny neurons (MSNs), yet their functional connectivity has been difficult to determine comprehensively. We generated a mouse with conditional channelrhodopsin-2 expression restricted to...

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Bibliographic Details
Published in:The Journal of neuroscience 2011-01, Vol.31 (4), p.1183-1192
Main Authors: Chuhma, Nao, Tanaka, Kenji F, Hen, René, Rayport, Stephen
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Channelrhodopsins
Corpus Striatum - physiology
Dendritic Spines - physiology
Dopamine - physiology
gamma-Aminobutyric Acid - physiology
Globus Pallidus - physiology
In Vitro Techniques
Inhibitory Postsynaptic Potentials
Interneurons - physiology
Mice
Mice, Transgenic
Neural Inhibition
Neurons - physiology
Patch-Clamp Techniques
Substantia Nigra - physiology
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Summary:Dopamine system disorders ranging from movement disorders to addiction and schizophrenia involve striatal medium spiny neurons (MSNs), yet their functional connectivity has been difficult to determine comprehensively. We generated a mouse with conditional channelrhodopsin-2 expression restricted to medium spiny neurons and assessed the specificity and strength of their intrinsic connections in the striatum and their projections to the globus pallidus and the substantia nigra. In the striatum, medium spiny neurons connected with other MSNs and tonically active cholinergic interneurons, but not with fast-spiking GABA interneurons. In the globus pallidus, medium spiny neurons connected strongly with one class of electrophysiologically identified neurons, but weakly with the other. In the substantia nigra, medium spiny neurons connected strongly with GABA, but not with dopamine neurons. Projections to the globus pallidus showed solely D2-mediated presynaptic inhibition, whereas projections to the substantia nigra showed solely D1-mediated presynaptic facilitation. This optogenetic approach defines the functional connectome of the striatal medium spiny neuron.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.3833-10.2011