Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing

Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman...

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Bibliographic Details
Published in:Immunogenetics (New York) 2010-12, Vol.62 (11-12), p.773-780
Main Authors: Budde, Melisa L, Wiseman, Roger W, Karl, Julie A, Hanczaruk, Bozena, Simen, Birgitte B, O'Connor, David H
Format: Article
Language:English
Subjects:
Allergology
Animal populations
Animals
Biomedical and Life Sciences
Biomedicine
Brief Communication
Cell Biology
Cloning
Cloning, Molecular
Cynomolgus
Gene Function
Genes, MHC Class I
Genomes
Haplotypes
Human Genetics
Immunology
Macaca
Macaca fascicularis - genetics
Macaca fascicularis - immunology
Macaca fasicularis
Mauritian cynomolgus macaque
MHC
Monkeys & apes
Primates
Pyrosequencing
Sequence Analysis, DNA
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Summary:Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-010-0481-9