On the rapid deprotection of synthetic oligonucleotides and analogs

The efficiency of ollgodeoxynucleotlde deprotection is greatly enhanced using a combination of: (a) ethanolamlne, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between...

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Bibliographic Details
Published in:Nucleic acids research 1994-02, Vol.22 (4), p.639-645
Main Authors: Polushin, Nikolai N., Morocho, Alan M., Chen, Ban-chin, Cohen, Jack S.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Chromatography, High Pressure Liquid
Enzymes and enzyme inhibitors
Ethanolamine
Ethanolamines - chemistry
Fundamental and applied biological sciences. Psychology
Hydrazines - chemistry
Molecular Sequence Data
Oligodeoxyribonucleotides - chemical synthesis
Oligonucleotides - chemical synthesis
Polymerase Chain Reaction
Thionucleotides - chemical synthesis
Time Factors
Transferases
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Summary:The efficiency of ollgodeoxynucleotlde deprotection is greatly enhanced using a combination of: (a) ethanolamlne, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotlde and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as In the case of ammonia deprotection. This method Is also shown to be applicable to the preparation of phosphorothloate and methylphosphonate oligodeoxynucleotldes and oligoribonucleotldes.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/22.4.639