VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering

We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of the...

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Bibliographic Details
Published in:Nucleic acids research 2011-04, Vol.39 (8), p.e49-e49
Main Authors: Suzuki, Emiko, Nakayama, Manabu
Format: Article
Language:English
Subjects:
Alleles
Animals
DNA - chemistry
Embryonic Stem Cells - metabolism
Genetic Engineering - methods
Genome
HEK293 Cells
Humans
Methods Online
Mice
Mutation
Recombinases - metabolism
Recombination, Genetic
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Summary:We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkq1280