Loading…

The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup

The aim of our work was to study apoptosis during the development of the retinal pigment epithelium (RPE) in mice between embryonic day (E) 10.5 and E12.5 and to examine a possible link between apoptosis and pigmentation. We collected mouse embryos at E10.5, E11.5, and E12.5 and labeled apoptotic ce...

Full description

Saved in:
Bibliographic Details
Published in:Molecular vision 2011-04, Vol.17, p.989-996
Main Authors: Pequignot, M O, Provost, A C, Sallé, S, Menasche, M, Saule, S, Jaïs, J-P, Abitbol, M
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 996
container_issue
container_start_page 989
container_title Molecular vision
container_volume 17
creator Pequignot, M O
Provost, A C
Sallé, S
Menasche, M
Saule, S
Jaïs, J-P
Abitbol, M
description The aim of our work was to study apoptosis during the development of the retinal pigment epithelium (RPE) in mice between embryonic day (E) 10.5 and E12.5 and to examine a possible link between apoptosis and pigmentation. We collected mouse embryos at E10.5, E11.5, and E12.5 and labeled apoptotic cells in 5-µm paraffin sections, using the terminal deoxynucleotidyl transferase dUTP nick end labeling technique. We counted the total number of cells and the number of apoptotic cells in the early developing RPE and calculated the percentage of apoptosis at each stage. In the C57BL/6J mouse, 17% of the RPE cells were apoptotic at E10.5 compared to 0.9% at E12.5. At E11.5, three-quarters of the RPE cells began to pigment, and apoptotic cells were located mostly in the nonpigmented part. In contrast, in the BALB/c mouse (tyrosinase-deficient) and pJ mouse (carrying mutations in the p gene) hypopigmented strains, the RPE contained significantly fewer apoptotic cells (7.5% and 10.1%, respectively) at E10.5 than controls. Subsequently at E11.5 and E12.5, the two hypopigmented strains displayed different apoptotic patterns; the BALB/c RPE had a similar percentage of apoptotic cells to controls (1.5% and 1.1%, respectively, for BALB/c versus 3.0% and 0.9%, respectively, for C57BL/6J), whereas the pJ RPE contained significantly more apoptosis (7.5% and 3.5%, respectively). Overall we observed differences in the evolution of the relative total number of RPE cells between the three strains. Apoptosis is a main event during the first stages of normal RPE development, indicating an essential role during RPE differentiation. Moreover, the early apoptotic pattern and possibly the whole early development of the RPE is different between hypopigmented and pigmented strains, as well as between BALB/c and pJ mice. This suggests the existence of regulatory and developmental differences with a more complex origin than just differing pigmentation levels.
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3084227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>923210283</sourcerecordid><originalsourceid>FETCH-LOGICAL-p297t-f585c0fbae9cd19d45c6f0f1a16567c3cdee67c3d47eb1b3f35543522beaa8513</originalsourceid><addsrcrecordid>eNpVkF1LwzAUhoMobk7_guTOq0I-mrW9EWT4BYI387qkyUkXaZOYtIP9ezu2ybx6D-fjeQ_vBZpTUpGMCC4uz-oZuknpmxBGRV5co9leKSv4HO3WG8ARButkh4Nte3ADhmCHDXR27PHoNMTWQ8K9TMluAcvgw-CTTViP0boWg4zdDmtrDMTp2srBeoel0yfeoeENnqB4OrYKqzHcoisjuwR3R12gr5fn9eot-_h8fV89fWSBVcWQGVEKRUwjoVKaVjoXammIoZIuxbJQXGmAveq8gIY23HAhci4Ya0DKUlC-QI8HbhibHrSaHoqyq0O0vYy72ktb_584u6lbv605KXM2hbRAD0dA9D8jpKHubVLQddKBH1NdMc4oYSWfNu_Prf48TnHzXwKhggM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>923210283</pqid></control><display><type>article</type><title>The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup</title><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Pequignot, M O ; Provost, A C ; Sallé, S ; Menasche, M ; Saule, S ; Jaïs, J-P ; Abitbol, M</creator><creatorcontrib>Pequignot, M O ; Provost, A C ; Sallé, S ; Menasche, M ; Saule, S ; Jaïs, J-P ; Abitbol, M</creatorcontrib><description>The aim of our work was to study apoptosis during the development of the retinal pigment epithelium (RPE) in mice between embryonic day (E) 10.5 and E12.5 and to examine a possible link between apoptosis and pigmentation. We collected mouse embryos at E10.5, E11.5, and E12.5 and labeled apoptotic cells in 5-µm paraffin sections, using the terminal deoxynucleotidyl transferase dUTP nick end labeling technique. We counted the total number of cells and the number of apoptotic cells in the early developing RPE and calculated the percentage of apoptosis at each stage. In the C57BL/6J mouse, 17% of the RPE cells were apoptotic at E10.5 compared to 0.9% at E12.5. At E11.5, three-quarters of the RPE cells began to pigment, and apoptotic cells were located mostly in the nonpigmented part. In contrast, in the BALB/c mouse (tyrosinase-deficient) and pJ mouse (carrying mutations in the p gene) hypopigmented strains, the RPE contained significantly fewer apoptotic cells (7.5% and 10.1%, respectively) at E10.5 than controls. Subsequently at E11.5 and E12.5, the two hypopigmented strains displayed different apoptotic patterns; the BALB/c RPE had a similar percentage of apoptotic cells to controls (1.5% and 1.1%, respectively, for BALB/c versus 3.0% and 0.9%, respectively, for C57BL/6J), whereas the pJ RPE contained significantly more apoptosis (7.5% and 3.5%, respectively). Overall we observed differences in the evolution of the relative total number of RPE cells between the three strains. Apoptosis is a main event during the first stages of normal RPE development, indicating an essential role during RPE differentiation. Moreover, the early apoptotic pattern and possibly the whole early development of the RPE is different between hypopigmented and pigmented strains, as well as between BALB/c and pJ mice. This suggests the existence of regulatory and developmental differences with a more complex origin than just differing pigmentation levels.</description><identifier>ISSN: 1090-0535</identifier><identifier>EISSN: 1090-0535</identifier><identifier>PMID: 21541273</identifier><language>eng</language><publisher>United States: Molecular Vision</publisher><subject>Albinism - genetics ; Amino Acid Substitution - genetics ; Animals ; Apoptosis ; Cell Differentiation ; Embryo, Mammalian - cytology ; Embryo, Mammalian - metabolism ; Female ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Monophenol Monooxygenase - genetics ; Monophenol Monooxygenase - metabolism ; Pigmentation - genetics ; Retinal Pigment Epithelium - cytology ; Retinal Pigment Epithelium - embryology ; Species Specificity</subject><ispartof>Molecular vision, 2011-04, Vol.17, p.989-996</ispartof><rights>Copyright © 2011 Molecular Vision. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084227/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084227/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21541273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pequignot, M O</creatorcontrib><creatorcontrib>Provost, A C</creatorcontrib><creatorcontrib>Sallé, S</creatorcontrib><creatorcontrib>Menasche, M</creatorcontrib><creatorcontrib>Saule, S</creatorcontrib><creatorcontrib>Jaïs, J-P</creatorcontrib><creatorcontrib>Abitbol, M</creatorcontrib><title>The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup</title><title>Molecular vision</title><addtitle>Mol Vis</addtitle><description>The aim of our work was to study apoptosis during the development of the retinal pigment epithelium (RPE) in mice between embryonic day (E) 10.5 and E12.5 and to examine a possible link between apoptosis and pigmentation. We collected mouse embryos at E10.5, E11.5, and E12.5 and labeled apoptotic cells in 5-µm paraffin sections, using the terminal deoxynucleotidyl transferase dUTP nick end labeling technique. We counted the total number of cells and the number of apoptotic cells in the early developing RPE and calculated the percentage of apoptosis at each stage. In the C57BL/6J mouse, 17% of the RPE cells were apoptotic at E10.5 compared to 0.9% at E12.5. At E11.5, three-quarters of the RPE cells began to pigment, and apoptotic cells were located mostly in the nonpigmented part. In contrast, in the BALB/c mouse (tyrosinase-deficient) and pJ mouse (carrying mutations in the p gene) hypopigmented strains, the RPE contained significantly fewer apoptotic cells (7.5% and 10.1%, respectively) at E10.5 than controls. Subsequently at E11.5 and E12.5, the two hypopigmented strains displayed different apoptotic patterns; the BALB/c RPE had a similar percentage of apoptotic cells to controls (1.5% and 1.1%, respectively, for BALB/c versus 3.0% and 0.9%, respectively, for C57BL/6J), whereas the pJ RPE contained significantly more apoptosis (7.5% and 3.5%, respectively). Overall we observed differences in the evolution of the relative total number of RPE cells between the three strains. Apoptosis is a main event during the first stages of normal RPE development, indicating an essential role during RPE differentiation. Moreover, the early apoptotic pattern and possibly the whole early development of the RPE is different between hypopigmented and pigmented strains, as well as between BALB/c and pJ mice. This suggests the existence of regulatory and developmental differences with a more complex origin than just differing pigmentation levels.</description><subject>Albinism - genetics</subject><subject>Amino Acid Substitution - genetics</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Differentiation</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Pigmentation - genetics</subject><subject>Retinal Pigment Epithelium - cytology</subject><subject>Retinal Pigment Epithelium - embryology</subject><subject>Species Specificity</subject><issn>1090-0535</issn><issn>1090-0535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpVkF1LwzAUhoMobk7_guTOq0I-mrW9EWT4BYI387qkyUkXaZOYtIP9ezu2ybx6D-fjeQ_vBZpTUpGMCC4uz-oZuknpmxBGRV5co9leKSv4HO3WG8ARButkh4Nte3ADhmCHDXR27PHoNMTWQ8K9TMluAcvgw-CTTViP0boWg4zdDmtrDMTp2srBeoel0yfeoeENnqB4OrYKqzHcoisjuwR3R12gr5fn9eot-_h8fV89fWSBVcWQGVEKRUwjoVKaVjoXammIoZIuxbJQXGmAveq8gIY23HAhci4Ya0DKUlC-QI8HbhibHrSaHoqyq0O0vYy72ktb_584u6lbv605KXM2hbRAD0dA9D8jpKHubVLQddKBH1NdMc4oYSWfNu_Prf48TnHzXwKhggM</recordid><startdate>20110420</startdate><enddate>20110420</enddate><creator>Pequignot, M O</creator><creator>Provost, A C</creator><creator>Sallé, S</creator><creator>Menasche, M</creator><creator>Saule, S</creator><creator>Jaïs, J-P</creator><creator>Abitbol, M</creator><general>Molecular Vision</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20110420</creationdate><title>The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup</title><author>Pequignot, M O ; Provost, A C ; Sallé, S ; Menasche, M ; Saule, S ; Jaïs, J-P ; Abitbol, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p297t-f585c0fbae9cd19d45c6f0f1a16567c3cdee67c3d47eb1b3f35543522beaa8513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Albinism - genetics</topic><topic>Amino Acid Substitution - genetics</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Differentiation</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Monophenol Monooxygenase - genetics</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Pigmentation - genetics</topic><topic>Retinal Pigment Epithelium - cytology</topic><topic>Retinal Pigment Epithelium - embryology</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pequignot, M O</creatorcontrib><creatorcontrib>Provost, A C</creatorcontrib><creatorcontrib>Sallé, S</creatorcontrib><creatorcontrib>Menasche, M</creatorcontrib><creatorcontrib>Saule, S</creatorcontrib><creatorcontrib>Jaïs, J-P</creatorcontrib><creatorcontrib>Abitbol, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular vision</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pequignot, M O</au><au>Provost, A C</au><au>Sallé, S</au><au>Menasche, M</au><au>Saule, S</au><au>Jaïs, J-P</au><au>Abitbol, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup</atitle><jtitle>Molecular vision</jtitle><addtitle>Mol Vis</addtitle><date>2011-04-20</date><risdate>2011</risdate><volume>17</volume><spage>989</spage><epage>996</epage><pages>989-996</pages><issn>1090-0535</issn><eissn>1090-0535</eissn><abstract>The aim of our work was to study apoptosis during the development of the retinal pigment epithelium (RPE) in mice between embryonic day (E) 10.5 and E12.5 and to examine a possible link between apoptosis and pigmentation. We collected mouse embryos at E10.5, E11.5, and E12.5 and labeled apoptotic cells in 5-µm paraffin sections, using the terminal deoxynucleotidyl transferase dUTP nick end labeling technique. We counted the total number of cells and the number of apoptotic cells in the early developing RPE and calculated the percentage of apoptosis at each stage. In the C57BL/6J mouse, 17% of the RPE cells were apoptotic at E10.5 compared to 0.9% at E12.5. At E11.5, three-quarters of the RPE cells began to pigment, and apoptotic cells were located mostly in the nonpigmented part. In contrast, in the BALB/c mouse (tyrosinase-deficient) and pJ mouse (carrying mutations in the p gene) hypopigmented strains, the RPE contained significantly fewer apoptotic cells (7.5% and 10.1%, respectively) at E10.5 than controls. Subsequently at E11.5 and E12.5, the two hypopigmented strains displayed different apoptotic patterns; the BALB/c RPE had a similar percentage of apoptotic cells to controls (1.5% and 1.1%, respectively, for BALB/c versus 3.0% and 0.9%, respectively, for C57BL/6J), whereas the pJ RPE contained significantly more apoptosis (7.5% and 3.5%, respectively). Overall we observed differences in the evolution of the relative total number of RPE cells between the three strains. Apoptosis is a main event during the first stages of normal RPE development, indicating an essential role during RPE differentiation. Moreover, the early apoptotic pattern and possibly the whole early development of the RPE is different between hypopigmented and pigmented strains, as well as between BALB/c and pJ mice. This suggests the existence of regulatory and developmental differences with a more complex origin than just differing pigmentation levels.</abstract><cop>United States</cop><pub>Molecular Vision</pub><pmid>21541273</pmid><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1090-0535
ispartof Molecular vision, 2011-04, Vol.17, p.989-996
issn 1090-0535
1090-0535
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3084227
source PubMed Central; Free Full-Text Journals in Chemistry
subjects Albinism - genetics
Amino Acid Substitution - genetics
Animals
Apoptosis
Cell Differentiation
Embryo, Mammalian - cytology
Embryo, Mammalian - metabolism
Female
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Monophenol Monooxygenase - genetics
Monophenol Monooxygenase - metabolism
Pigmentation - genetics
Retinal Pigment Epithelium - cytology
Retinal Pigment Epithelium - embryology
Species Specificity
title The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T11%3A59%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20retinal%20pigment%20epithelium%20undergoes%20massive%20apoptosis%20during%20early%20differentiation%20and%20pigmentation%20of%20the%20optic%20cup&rft.jtitle=Molecular%20vision&rft.au=Pequignot,%20M%20O&rft.date=2011-04-20&rft.volume=17&rft.spage=989&rft.epage=996&rft.pages=989-996&rft.issn=1090-0535&rft.eissn=1090-0535&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E923210283%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p297t-f585c0fbae9cd19d45c6f0f1a16567c3cdee67c3d47eb1b3f35543522beaa8513%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=923210283&rft_id=info:pmid/21541273&rfr_iscdi=true