Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most si...

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Bibliographic Details
Published in:Biotechnology research international 2011-03, Vol.2011 (2011), p.1-7
Main Authors: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
Format: Article
Language:English
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Summary:Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH4NO3—6.63 mg/gds, MnSO4⋅7H2O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K2HPO4—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.
ISSN:2090-3138
2090-3146
DOI:10.4061/2011/708043