PREPARATIVE ISOLATION AND PURIFICATION OF CHEMICAL CONSTITUENTS OF BELAMCANDA BY MPLC, HSCCC, AND PREP-HPLC

Combined with medium-pressure liquid chromatography (MPLC) and preparative high-pressure liquid chromatography (Prep-HPLC), high-speed countercurrent chromatography (HSCCC) was successfully applied for separation and purification of isoflavonoids from the extract of belamcanda. HSCCC separation was...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2011-01, Vol.34 (4), p.241-257
Main Authors: Wang, Xiaohong, Liang, Yong, Peng, Cuilin, Xie, Huichun, Pan, Man, Zhang, Tianyou, Ito, Yoichiro
Format: Article
Language:English
Subjects:
Belamcanda
Biological and medical sciences
General pharmacology
high-speed countercurrent chromatography
isoflavonoids
Medical sciences
medium-pressure liquid chromatography
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
preparative high-pressure liquid chromatography
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Summary:Combined with medium-pressure liquid chromatography (MPLC) and preparative high-pressure liquid chromatography (Prep-HPLC), high-speed countercurrent chromatography (HSCCC) was successfully applied for separation and purification of isoflavonoids from the extract of belamcanda. HSCCC separation was performed on a two-phase solvent system composed of methyl tert-butyl ether-ethyl acetate-n-butyl alcohol-acetonitrile-0.1% aqueous trifluoroacetic acid at a volume radio of 1:2:1:1:5. Semi-purified peak fractions from HSCCC separation were further purified by Prep-HPLC. Nine well-separated fractions were analyzed by HPLC-UV absorption spectrometry to determine their purities and characterized with ESI-MS n . Except for peaks I and VII (unknown), seven compounds were identified as apocynin (peak II), mangiferin (peak III), 7-O-methylmangiferin (peak IV), hispidulin (peak V), 3′-hydroxyltectoridin (peak VI), iristectorin B (peak VIII), and isoiridin (peak IX).
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2011.547058