Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the...

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Bibliographic Details
Published in:Biomedical optics express 2011-04, Vol.2 (5), p.1359-1365
Main Authors: Liu, Tan, Wei, Qing, Wang, Jing, Jiao, Shuliang, Zhang, Hao F
Format: Article
Language:English
Subjects:
Multimodal Imaging
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Summary:We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO(2) by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO(2) were further calculated. The measured blood flow rates in the two vessels agreed with each other.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.2.001359