Location and architecture of the Caulobacter crescentus chemoreceptor array

Summary A new method for recording both fluorescence and cryo‐EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. We show that in wild‐type cells preserved in a near‐native state, the chemoreceptors are h...

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Bibliographic Details
Published in:Molecular microbiology 2008-07, Vol.69 (1), p.30-41
Main Authors: Briegel, Ariane, Ding, H. Jane, Li, Zhuo, Werner, John, Gitai, Zemer, Dias, D. Prabha, Jensen, Rasmus B., Jensen, Grant J.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - ultrastructure
Bacteriology
Biological and medical sciences
Caulobacter crescentus
Caulobacter crescentus - chemistry
Caulobacter crescentus - genetics
Caulobacter crescentus - metabolism
Caulobacter crescentus - ultrastructure
Cells
Chemoreceptor Cells - chemistry
Chemoreceptor Cells - metabolism
Chemoreceptor Cells - ultrastructure
Flagella - chemistry
Flagella - genetics
Flagella - metabolism
Flagella - ultrastructure
Flagellin - chemistry
Flagellin - genetics
Flagellin - metabolism
Flagellin - ultrastructure
Fluorescence
Fundamental and applied biological sciences. Psychology
Image Processing, Computer-Assisted
Microbiology
Miscellaneous
Mutation
Protein Array Analysis - methods
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Summary:Summary A new method for recording both fluorescence and cryo‐EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. We show that in wild‐type cells preserved in a near‐native state, the chemoreceptors are hexagonally packed with a lattice spacing of 12 nm, just a few tens of nanometers away from the flagellar motor that they control. The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. Placing the known crystal structure of a trimer of receptor dimers at each vertex of the lattice accounts well for the density and agrees with other constraints. Based on this model for the arrangement of receptors, there are between one and two thousand receptors per array.
ISSN:0950-382X
1365-2958
DOI:10.1111/j.1365-2958.2008.06219.x