Detection of UV-induced RNA: protein crosslinks in snRNPs by oligonucleotides complementary to the snRNA

Covalent crosslinking of proteins to RNA followed by analysis on SDS-polyacrylamide gels is commonly used as a method to characterise ribonucleoprotein complexes. However this method usually requires either extensive purification of the complex and labelling of the RNA, detection of the RNA by hybri...

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Bibliographic Details
Published in:Nucleic acids research 1993-02, Vol.21 (4), p.1049-1050
Main Authors: Mital, Renu, Albrecht, Urs, Schümperli, Daniel
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Molecular Weight
Nucleic Acid Hybridization
Nucleic acids
Oligonucleotides - chemistry
Ribonucleoproteins, Small Nuclear - chemistry
Ribonucleoproteins, Small Nuclear - radiation effects
Rna, ribonucleoproteins
RNA, Small Nuclear - chemistry
Ultraviolet Rays
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Summary:Covalent crosslinking of proteins to RNA followed by analysis on SDS-polyacrylamide gels is commonly used as a method to characterise ribonucleoprotein complexes. However this method usually requires either extensive purification of the complex and labelling of the RNA, detection of the RNA by hybridisation after blotting, or the ability to form the complex in vivo with radioactively labelled RNA. We show here that these problems can be overcome if the RNA in the crosslinked products is detected by hybridisation with a short radioactively labelled complementary oligonucleotide.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.4.1049