Rat and chick cDNA clones encoding HMG-like proteins

HMG box containing proteins, such as hUBF and SRY, bind DNA and may regulate transcription; however, a consensus binding site has not been identified. The mouse HMG-like protein (T160) binds to a sequence involved in V-(D)-J recombination, whereas the homologous human protein (SSRP1) binds to cispla...

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Bibliographic Details
Published in:Nucleic acids research 1993-03, Vol.21 (6), p.1493-1493
Main Authors: Wang, L, Precht, P, Balakir, R, Horton, W.E. Jr
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Binding and carrier proteins
binding sites
Biological and medical sciences
Chickens
Cloning, Molecular
complementary DNA
DNA - genetics
DNA-binding proteins
Fundamental and applied biological sciences. Psychology
genbank/l08814
genbank/l08815
high mobility group proteins
High Mobility Group Proteins - genetics
Humans
messenger RNA
Mice
Molecular Sequence Data
nucleotide sequences
Oligodeoxyribonucleotides - chemistry
Proteins
Rats
Sequence Alignment
Sequence Homology, Amino Acid
transcription (genetics)
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Summary:HMG box containing proteins, such as hUBF and SRY, bind DNA and may regulate transcription; however, a consensus binding site has not been identified. The mouse HMG-like protein (T160) binds to a sequence involved in V-(D)-J recombination, whereas the homologous human protein (SSRP1) binds to cisplatin modified DNA. We have cloned a 2.1 kb rat cDNA (CIIDBP-r) by screening a chondrosarcoma cDNA expression library with a sequence (5'-TCTCCCAC-AATGCATCCCCC-3') located in the first intron enhancer of the collagen II gene that has been shown to bind chondrocyte nuclear factors. We then isolated a chick homolog (2.1 kb, CIIDBP-c) by Southern screening of a chick chondrocyte cDNA library. Comparison of the DNA and derived protein sequences revealed that the CIIDBP-r and CIIDBP-c are highly homologous to each other and to T160 and SSRP1. A comparison of the sequence within the HMG box (the putative DNA binding domain) suggests higher conservation between mammals than between mammalian and avian species. Also, the identity within the HMG box between the HMG-like proteins and that of HMG-1 is less than 50%, suggesting that they may be evolutionary linked but functionally distinct. Probing of Northern blots with both the rat and chick cDNAs revealed the expression of a 2.3 kb transcript in a wide variety of cell types (data not shown), as is the case for both the mouse and human clones as well.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.6.1493