Transcription of human 5S rRNA genes is influenced by an upstream DNA sequence

Six human 5S rRNA genes and gene variants and one pseudogene have been sequenced. The six genes/variants were transcribed In a HeLa cell extract with about equal efficiency. Three genes contain the Sp1 binding sequence GGGCGG in position -43 to -38 and three genes contain the Sp1 like sequence GGGCC...

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Bibliographic Details
Published in:Nucleic acids research 1993-08, Vol.21 (16), p.3631-3636
Main Authors: Nielsen, Jens Nederby, Hallenberg, Charlotte, Frederiksen, Sune, Sørensen, Pernille Dissing, Lomholt, Bodil
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cloning, Molecular
Cricetinae
DNA
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Protein Binding
Pseudogenes
Regulatory Sequences, Nucleic Acid
Restriction Mapping
RNA, Ribosomal, 5S - genetics
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:Six human 5S rRNA genes and gene variants and one pseudogene have been sequenced. The six genes/variants were transcribed In a HeLa cell extract with about equal efficiency. Three genes contain the Sp1 binding sequence GGGCGG in position -43 to -38 and three genes contain the Sp1 like sequence GGGCCG In this position. The six genes contain furthermore one Sp1 binding site in a position about -245 and one ATF recognition site in a position about -202. A 12 bp sequence (GGCTCTTGGGGC) found in position -32 to -21 strongly influenced the transcriptlonal efficiency in vitro. This 12-mer, designated the D box, has also been found upstream a 5S rRNA gene from hamster and mouse. Removal of the Sp1 binding sites had no effect on the transcription in vitro whereas the transcriptlonal efficiency decreased to 10% If the D vox was removed from the human 5S rRNA gene.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.16.3631