Construction of random small-insert genomic libraries highly enriched for simple sequence repeats

We present an efficient method for construction of random small-insert genomic libraries enriched for (CA) sub(n) repeats. The method involves fragmentation of DNA by sonication and ligation of an adaptor to the fragments followed by PCR amplification and enrichment for (CA) sub(n) repeats by hybrid...

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Bibliographic Details
Published in:Nucleic acids research 1993-08, Vol.21 (16), p.3911-3912
Main Authors: Karagyozov, L, Kalcheva, I.D, Chapman, V.M
Format: Article
Language:English
Subjects:
Animals
Base Sequence
biochemical techniques
Biological and medical sciences
Cloning, Molecular
dinucleotide repeat sequences
Diverse techniques
DNA
DNA libraries
Fundamental and applied biological sciences. Psychology
Genomic Library
Humans
Mice
Molecular and cellular biology
Molecular Sequence Data
nucleic acid hybridization
Polymerase Chain Reaction
repetitive sequences
Repetitive Sequences, Nucleic Acid
Sonication
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Summary:We present an efficient method for construction of random small-insert genomic libraries enriched for (CA) sub(n) repeats. The method involves fragmentation of DNA by sonication and ligation of an adaptor to the fragments followed by PCR amplification and enrichment for (CA) sub(n) repeats by hybridization to (GT) sub(15) Oligonucleotides bound to a nylon membrane.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.16.3911