fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth

Only four prp (pre-mRNA processing) genes of the fission yeast Schizosaccharomyces pombe have been reported. We exploited yeast genetics and identified and isolated the prp4 gene. Sequence analysis revealed that the splicing factor encoded by this gene contains the signature sequences that define th...

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Bibliographic Details
Published in:Nucleic acids research 1993-08, Vol.21 (17), p.4079-4083
Main Authors: Alahari, S.K, Schmidt, H, Kaufer, N.F
Format: Article
Language:English
Subjects:
alternative splicing
Amino Acid Sequence
amino acid sequences
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Southern
DNA, Fungal
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
genbank/l10739
Genes, Fungal
Genes. Genome
Genetic Complementation Test
Introns
messenger RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequences
precursors
Protein-Serine-Threonine Kinases - genetics
Restriction Mapping
Ribonucleoprotein, U4-U6 Small Nuclear - genetics
RNA Precursors - metabolism
RNA Splicing - genetics
Saccharomycetales
Schizosaccharomyces - enzymology
Schizosaccharomyces - genetics
Schizosaccharomyces - growth & development
Schizosaccharomyces pombe
Sequence Homology, Amino Acid
serine
structural genes
Suppression, Genetic
threonine
Transcription, Genetic
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Description
Summary:Only four prp (pre-mRNA processing) genes of the fission yeast Schizosaccharomyces pombe have been reported. We exploited yeast genetics and identified and isolated the prp4 gene. Sequence analysis revealed that the splicing factor encoded by this gene contains the signature sequences that define the serine/threonine protein kinase family. This is the first kinase gene identified whose product is involved in pre-mRNA splicing. The prp4 gene contains one intron in the kinase domain. Gene replacement studies provided evidence that this gene is essential for growth and is located on chromosome III.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.17.4079