Identification and Characterization of a Transcriptional Silencer Upstream of the Human BRCA2 Gene

Expression of the breast cancer susceptibility tumor-suppressor protein BRCA2, a protein potentially involved in DNA recombination repair, is tightly regulated throughout development. We have identified a transcriptional silencer at the distal end of the human BRCA2 gene promoter. This silencer is i...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-11, Vol.265 (2), p.285-290
Main Authors: Sharan, Chakradhari, Hamilton, Nalo M., Parl, Angelika K., Singh, Pramod K., Chaudhuri, Gautam
Format: Article
Language:English
Subjects:
Alu Elements
Amino Acid Sequence
Base Sequence
BRCA2 Protein
Breast - metabolism
Breast Neoplasms - genetics
Cell Line
DNA - genetics
DNA - metabolism
DNA Primers - genetics
DNA-Binding Proteins - metabolism
Female
Gene Expression Regulation, Neoplastic
Genes, Tumor Suppressor
HeLa Cells
Humans
Molecular Sequence Data
Neoplasm Proteins - genetics
Promoter Regions, Genetic
Sequence Deletion
Spermatozoa
Transcription Factors - genetics
Tumor Cells, Cultured
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Summary:Expression of the breast cancer susceptibility tumor-suppressor protein BRCA2, a protein potentially involved in DNA recombination repair, is tightly regulated throughout development. We have identified a transcriptional silencer at the distal end of the human BRCA2 gene promoter. This silencer is involved in the negative regulation of the expression of this gene in breast cell lines tested but not in HeLa or HepG2 cells. The 221-base-pair silencer region is characterized by a full-length Alu-repeat. Presence of specific BRCA2 silencer-binding proteins in the breast cell extracts indicates the potential regulation of BRCA2 gene expression by these proteins.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1652