Solid-phase extraction and GC-MS analysis of potentially genotoxic cleavage products of β-carotene in primary cell cultures

• Published in: Analytical and bioanalytical chemistry 2011-06, Vol.400 (8), p.2415-2426
• Main Authors: Martano, G., Vogl, C., Bojaxhi, E., Bresgen, N., Eckl, P., Stutz, H.
• Format: Article
• Language: English
• Subjects: Alveolar Epithelial Cells - chemistry; Alveolar Epithelial Cells - cytology; Analytical Chemistry; Animals; Beta carotene; beta Carotene - analysis; Biochemistry; Cells, Cultured; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Food Science; Gas Chromatography-Mass Spectrometry; Hepatocytes - chemistry; Hepatocytes - cytology; Ionization; Laboratory Medicine; Mass spectrometry; Molecular Structure; Monitoring/Environmental Analysis; Original Paper; Rats; Solid Phase Extraction; Stereoisomerism
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-011-4836-3

A validated method for the simultaneous determination of prominent volatile cleavage products (CPs) of β-carotene in cell culture media has been developed. Target CPs comprised β-ionone (β-IO), cyclocitral (CC), dihydroactinidiolide (DHA), and 1,1,6-trimethyltetraline (TMT). CPs were extracted by solid-phase extraction applying a phenyl adsorbent, eluted with 10% ( v / v ) tetrahydrofuran in n -hexane, and identified and quantified by gas chromatography-mass spectrometry with electron impact ionization. Method validation addressed linearity confirmation over two application ranges and homoscedasticity testing. Recoveries from culture media were between 71.7% and 95.7% at 1.0 μg/ml. Precision of recoveries determined in intra-day ( N  = 5) and inter-day ( N  = 15) assays were